Next Article in Journal
Acknowledgement to Reviewers of Metabolites in 2017
Next Article in Special Issue
Global Metabolomics of the Placenta Reveals Distinct Metabolic Profiles between Maternal and Fetal Placental Tissues Following Delivery in Non-Labored Women
Previous Article in Journal
Calcitriol Supplementation Causes Decreases in Tumorigenic Proteins and Different Proteomic and Metabolomic Signatures in Right versus Left-Sided Colon Cancer
Previous Article in Special Issue
Integrated Metabolomics Assessment of Human Dried Blood Spots and Urine Strips
Article Menu
Issue 1 (March) cover image

Export Article

Open AccessArticle
Metabolites 2018, 8(1), 6; https://doi.org/10.3390/metabo8010006

Impact of Prolonged Blood Incubation and Extended Serum Storage at Room Temperature on the Human Serum Metabolome

1
Metanomics Health GmbH, Tegeler Weg 33, 10589 Berlin, Germany
2
Metanomics GmbH, Tegeler Weg 33, 10589 Berlin, Germany
3
Precision Medicine Unit, Precision Medicine and Genomics, IMED Biotech Unit, AstraZeneca, 43183 Mölndal, Gothenburg, Sweden
*
Author to whom correspondence should be addressed.
Received: 24 November 2017 / Revised: 5 January 2018 / Accepted: 11 January 2018 / Published: 13 January 2018
(This article belongs to the Special Issue Clinical Metabolomics)
Full-Text   |   PDF [2471 KB, uploaded 13 January 2018]   |  

Abstract

Metabolomics is a powerful technology with broad applications in life science that, like other -omics approaches, requires high-quality samples to achieve reliable results and ensure reproducibility. Therefore, along with quality assurance, methods to assess sample quality regarding pre-analytical confounders are urgently needed. In this study, we analyzed the response of the human serum metabolome to pre-analytical variations comprising prolonged blood incubation and extended serum storage at room temperature by using gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) -based metabolomics. We found that the prolonged incubation of blood results in a statistically significant 20% increase and 4% decrease of 225 tested serum metabolites. Extended serum storage affected 21% of the analyzed metabolites (14% increased, 7% decreased). Amino acids and nucleobases showed the highest percentage of changed metabolites in both confounding conditions, whereas lipids were remarkably stable. Interestingly, the amounts of taurine and O-phosphoethanolamine, which have both been discussed as biomarkers for various diseases, were 1.8- and 2.9-fold increased after 6 h of blood incubation. Since we found that both are more stable in ethylenediaminetetraacetic acid (EDTA) blood, EDTA plasma should be the preferred metabolomics matrix. View Full-Text
Keywords: biomarker; pre-analytical phase; serum; metabolomics; quality control; biobanking; mass spectrometry biomarker; pre-analytical phase; serum; metabolomics; quality control; biobanking; mass spectrometry
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Kamlage, B.; Neuber, S.; Bethan, B.; González Maldonado, S.; Wagner-Golbs, A.; Peter, E.; Schmitz, O.; Schatz, P. Impact of Prolonged Blood Incubation and Extended Serum Storage at Room Temperature on the Human Serum Metabolome. Metabolites 2018, 8, 6.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Metabolites EISSN 2218-1989 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top