Distribution of Heparan Sulfate Oligosaccharides in Murine Mucopolysaccharidosis Type IIIA
Abstract
:1. Introduction
2. Results
2.1. UA in Mouse Tissues and Urine
2.2. Distribution of Di- to Hexasaccharides in Mouse Tissues and Urine
Structure of oligosaccharide b | Brain c | Spleen c | Lung c | Heart c | Liver c | Kidney c | Average for all Tissues | Urine c |
---|---|---|---|---|---|---|---|---|
GlcN-UA (+S) | 5.0 | 4.8 | 4.6 | 5.2 | 6.0 | 3.7 | 5.0 | 2.1 |
GlcN-UA-GlcNAc (+2S) | 1.1 | 0.7 | 0.6 | 0.6 | 0.9 | 0.8 | 0.8 | 0.9 |
GlcN-UA-GlcN-UA (+3S) | 0.4 | 0.4 | 0.5 | 0.4 | 0.5 | 0.4 | 0.4 | 1.1 |
GlcN-UA-GlcNAc-UA (+2S) | 56.1 | 75.2 | 62.7 | 58.2 | 65.6 | 61.3 | 63.2 | 64.4 |
GlcN-UA-GlcN-UA-GlcNAc (+3 to+5S) | 8.9 | 2.5 | 3.7 | 5.2 | 4.2 | 6.1 | 5.0 | 3.4 |
GlcN-[UA-GlcN]/[UA-GlcNAc]-UA (+3 to +5S) | 28.5 | 16.4 | 27.9 | 30.4 | 22.8 | 27.7 | 25.6 | 28.0 |
2.3. Correlation between HS Oligosaccharides and UA in MPS IIIA Mouse Tissues
3. Experimental Section
3.1. Materials
3.2. Animals
3.3. Isolation of Oligosaccharides from Tissues and Urine
3.4. Total UA in Tissues and Urine
3.5. Derivatization of Oligosaccharides
3.6. HPLC ESI-MS/MS Analysis of di- to Hexasaccharides
3.7. Statistical Analysis
4. Discussion
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Mason, K.; Meikle, P.; Hopwood, J.; Fuller, M. Distribution of Heparan Sulfate Oligosaccharides in Murine Mucopolysaccharidosis Type IIIA. Metabolites 2014, 4, 1088-1100. https://doi.org/10.3390/metabo4041088
Mason K, Meikle P, Hopwood J, Fuller M. Distribution of Heparan Sulfate Oligosaccharides in Murine Mucopolysaccharidosis Type IIIA. Metabolites. 2014; 4(4):1088-1100. https://doi.org/10.3390/metabo4041088
Chicago/Turabian StyleMason, Kerryn, Peter Meikle, John Hopwood, and Maria Fuller. 2014. "Distribution of Heparan Sulfate Oligosaccharides in Murine Mucopolysaccharidosis Type IIIA" Metabolites 4, no. 4: 1088-1100. https://doi.org/10.3390/metabo4041088