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Metabolites 2014, 4(2), 453-464; doi:10.3390/metabo4020453

Surveillance of C-Allocation in Microalgal Cells

1,* , 1,2
1 Universität Leipzig, Institute of Biology–Plant Physiology, Johannisallee 23, 04103 Leipzig, Germany 2 Department of Computational Landscape Ecology, Helmholtz Centre for Environmental Research–UFZ, Permoserstraße 15, 04318 Leipzig, Germany
* Author to whom correspondence should be addressed.
Received: 29 April 2014 / Revised: 6 June 2014 / Accepted: 9 June 2014 / Published: 19 June 2014
(This article belongs to the Special Issue Metabolism in Phototrophic Prokaryotes and Algae)
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When microalgae are exposed to changing environmental conditions, e.g., light-dark cycles or oscillations in nutrient availability (CO2, nitrogen, phosphate or silicate) they respond with metabolic changes in the carbon allocation pattern. Short time regulations in the time range of few seconds to minutes can be mirrored best by mass spectroscopy based metabolomics. However, these snap shots do not reflect the alterations in the carbon flow to the cellular macromolecules like protein, carbohydrate or lipid. In this review it is shown how the combination of FTIR spectroscopy and Chla-in-vivo-fluorescence based electron transport rates can reveal changes in the metabolic flux rates of carbon during a shift of the environmental conditions. The review will demonstrate in which time range FTIR spectroscopy can deliver significant information and how FTIR spectroscopy data can synergistically support metabolome analysis by mass-spectroscopy.
Keywords: FTIR spectroscopy; algae; biomass; composition; metabolomics; chemometrics; Chlamydomonas FTIR spectroscopy; algae; biomass; composition; metabolomics; chemometrics; Chlamydomonas
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Wagner, H.; Jungandreas, A.; Fanesi, A.; Wilhelm, C. Surveillance of C-Allocation in Microalgal Cells. Metabolites 2014, 4, 453-464.

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