Surveillance of C-Allocation in Microalgal Cells
AbstractWhen microalgae are exposed to changing environmental conditions, e.g., light-dark cycles or oscillations in nutrient availability (CO2, nitrogen, phosphate or silicate) they respond with metabolic changes in the carbon allocation pattern. Short time regulations in the time range of few seconds to minutes can be mirrored best by mass spectroscopy based metabolomics. However, these snap shots do not reflect the alterations in the carbon flow to the cellular macromolecules like protein, carbohydrate or lipid. In this review it is shown how the combination of FTIR spectroscopy and Chla-in-vivo-fluorescence based electron transport rates can reveal changes in the metabolic flux rates of carbon during a shift of the environmental conditions. The review will demonstrate in which time range FTIR spectroscopy can deliver significant information and how FTIR spectroscopy data can synergistically support metabolome analysis by mass-spectroscopy.
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Wagner, H.; Jungandreas, A.; Fanesi, A.; Wilhelm, C. Surveillance of C-Allocation in Microalgal Cells. Metabolites 2014, 4, 453-464.
Wagner H, Jungandreas A, Fanesi A, Wilhelm C. Surveillance of C-Allocation in Microalgal Cells. Metabolites. 2014; 4(2):453-464.Chicago/Turabian Style
Wagner, Heiko; Jungandreas, Anne; Fanesi, Andrea; Wilhelm, Christian. 2014. "Surveillance of C-Allocation in Microalgal Cells." Metabolites 4, no. 2: 453-464.