Metabolites 2014, 4(2), 319-346; doi:10.3390/metabo4020319
Review

MALDI Mass Spectrometry Imaging for Visualizing In Situ Metabolism of Endogenous Metabolites and Dietary Phytochemicals

* email and * email
Received: 26 February 2014; in revised form: 17 April 2014 / Accepted: 4 May 2014 / Published: 9 May 2014
(This article belongs to the Special Issue Metabolomics and Biotechnology)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Understanding the spatial distribution of bioactive small molecules is indispensable for elucidating their biological or pharmaceutical roles. Mass spectrometry imaging (MSI) enables determination of the distribution of ionizable molecules present in tissue sections of whole-body or single heterogeneous organ samples by direct ionization and detection. This emerging technique is now widely used for in situ label-free molecular imaging of endogenous or exogenous small molecules. MSI allows the simultaneous visualization of many types of molecules including a parent molecule and its metabolites. Thus, MSI has received much attention as a potential tool for pathological analysis, understanding pharmaceutical mechanisms, and biomarker discovery. On the other hand, several issues regarding the technical limitations of MSI are as of yet still unresolved. In this review, we describe the capabilities of the latest matrix-assisted laser desorption/ionization (MALDI)-MSI technology for visualizing in situ metabolism of endogenous metabolites or dietary phytochemicals (food factors), and also discuss the technical problems and new challenges, including MALDI matrix selection and metabolite identification, that need to be addressed for effective and widespread application of MSI in the diverse fields of biological, biomedical, and nutraceutical (food functionality) research.
Keywords: mass spectrometry imaging; small molecule; metabolite; phytochemical; food functionality; metabolomics; MALDI; metabolite identification; QSPR
PDF Full-text Download PDF Full-Text [2566 KB, Updated Version, uploaded 13 May 2014 11:46 CEST]
The original version is still available [2693 KB, uploaded 9 May 2014 13:34 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Fujimura, Y.; Miura, D. MALDI Mass Spectrometry Imaging for Visualizing In Situ Metabolism of Endogenous Metabolites and Dietary Phytochemicals. Metabolites 2014, 4, 319-346.

AMA Style

Fujimura Y, Miura D. MALDI Mass Spectrometry Imaging for Visualizing In Situ Metabolism of Endogenous Metabolites and Dietary Phytochemicals. Metabolites. 2014; 4(2):319-346.

Chicago/Turabian Style

Fujimura, Yoshinori; Miura, Daisuke. 2014. "MALDI Mass Spectrometry Imaging for Visualizing In Situ Metabolism of Endogenous Metabolites and Dietary Phytochemicals." Metabolites 4, no. 2: 319-346.

Metabolites EISSN 2218-1989 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert