Acylcarnitine Profiles in Acetaminophen Toxicity in the Mouse: Comparison to Toxicity, Metabolism and Hepatocyte Regeneration
Abstract
:1. Introduction
2. Results and Discussion
2.1. Indicators of Hepatotoxicity and Oxidative Metabolism in APAP Treated Mice
2.2. Indicators of Hepatocyte Regeneration in APAP Toxicity
2.3. Oil Red O (ORO) Analysis and Liver Triglycerides
2.4. Acylcarnitine Profiles in APAP Toxicity in the Mouse
2.5. Relationship of Acylcarnitines to APAP Protein Adducts and Hepatic Glutathione
3. Materials and Methods
3.1. Drugs and Reagents
3.2. Experimental Animals
3.3. Assessment of Hepatotoxicity
3.4. Determination of Hepatic Glutathione and APAP Protein Adducts
3.5. Oil Red O Analysis of Hepatic Lipid Deposition
3.6. Proliferating Cell Nuclear Antigen (PCNA) Immunohistochemistry
3.7. Targeted Analysis of Serum Acylcarnitines Using a UPLC-TQ
3.8. Data Analysis
4. Concluding Remarks
Acknowledgments
Conflict of Interest
References
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Bhattacharyya, S.; Pence, L.; Beger, R.; Chaudhuri, S.; McCullough, S.; Yan, K.; Simpson, P.; Hennings, L.; Hinson, J.; James, L. Acylcarnitine Profiles in Acetaminophen Toxicity in the Mouse: Comparison to Toxicity, Metabolism and Hepatocyte Regeneration. Metabolites 2013, 3, 606-622. https://doi.org/10.3390/metabo3030606
Bhattacharyya S, Pence L, Beger R, Chaudhuri S, McCullough S, Yan K, Simpson P, Hennings L, Hinson J, James L. Acylcarnitine Profiles in Acetaminophen Toxicity in the Mouse: Comparison to Toxicity, Metabolism and Hepatocyte Regeneration. Metabolites. 2013; 3(3):606-622. https://doi.org/10.3390/metabo3030606
Chicago/Turabian StyleBhattacharyya, Sudeepa, Lisa Pence, Richard Beger, Shubhra Chaudhuri, Sandra McCullough, Ke Yan, Pippa Simpson, Leah Hennings, Jack Hinson, and Laura James. 2013. "Acylcarnitine Profiles in Acetaminophen Toxicity in the Mouse: Comparison to Toxicity, Metabolism and Hepatocyte Regeneration" Metabolites 3, no. 3: 606-622. https://doi.org/10.3390/metabo3030606