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Metabolites 2013, 3(2), 347-372; doi:10.3390/metabo3020347
Article

Metabolic and Transcriptional Reprogramming in Developing Soybean (Glycine max) Embryos

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Received: 15 March 2013; in revised form: 16 April 2013 / Accepted: 7 May 2013 / Published: 14 May 2013
(This article belongs to the Special Issue Metabolomics in Plant Metabolic Engineering)
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Abstract: Soybean (Glycine max) seeds are an important source of seed storage compounds, including protein, oil, and sugar used for food, feed, chemical, and biofuel production. We assessed detailed temporal transcriptional and metabolic changes in developing soybean embryos to gain a systems biology view of developmental and metabolic changes and to identify potential targets for metabolic engineering. Two major developmental and metabolic transitions were captured enabling identification of potential metabolic engineering targets specific to seed filling and to desiccation. The first transition involved a switch between different types of metabolism in dividing and elongating cells. The second transition involved the onset of maturation and desiccation tolerance during seed filling and a switch from photoheterotrophic to heterotrophic metabolism. Clustering analyses of metabolite and transcript data revealed clusters of functionally related metabolites and transcripts active in these different developmental and metabolic programs. The gene clusters provide a resource to generate predictions about the associations and interactions of unknown regulators with their targets based on “guilt-by-association” relationships. The inferred regulators also represent potential targets for future metabolic engineering of relevant pathways and steps in central carbon and nitrogen metabolism in soybean embryos and drought and desiccation tolerance in plants.
Keywords: central carbon and nitrogen metabolism; plant metabolic engineering; RNA sequencing; seed storage compounds; soybean; systems biology; transcriptomics; untargeted and targeted metabolomics central carbon and nitrogen metabolism; plant metabolic engineering; RNA sequencing; seed storage compounds; soybean; systems biology; transcriptomics; untargeted and targeted metabolomics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Collakova, E.; Aghamirzaie, D.; Fang, Y.; Klumas, C.; Tabataba, F.; Kakumanu, A.; Myers, E.; Heath, L.S.; Grene, R. Metabolic and Transcriptional Reprogramming in Developing Soybean (Glycine max) Embryos. Metabolites 2013, 3, 347-372.

AMA Style

Collakova E, Aghamirzaie D, Fang Y, Klumas C, Tabataba F, Kakumanu A, Myers E, Heath LS, Grene R. Metabolic and Transcriptional Reprogramming in Developing Soybean (Glycine max) Embryos. Metabolites. 2013; 3(2):347-372.

Chicago/Turabian Style

Collakova, Eva; Aghamirzaie, Delasa; Fang, Yihui; Klumas, Curtis; Tabataba, Farzaneh; Kakumanu, Akshay; Myers, Elijah; Heath, Lenwood S.; Grene, Ruth. 2013. "Metabolic and Transcriptional Reprogramming in Developing Soybean (Glycine max) Embryos." Metabolites 3, no. 2: 347-372.



Metabolites EISSN 2218-1989 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert