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• Ditlev, DBruun
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Metabolites 2013, 3(1), 33-46; doi:10.3390/metabo3010033

Article

Metabonomic Response to Milk Proteins after a Single Bout of Heavy Resistance Exercise Elucidated by ¹H Nuclear Magnetic Resonance Spectroscopy

Christian Clement Yde ^1,* , Ditte Bruun Ditlev ¹ , Søren Reitelseder ²  and Hanne Christine Bertram ¹

¹ Department of Food Science, Aarhus University, Aarslev, Denmark ² Institute of Sports Medicine Copenhagen, Department of Orthopedic Surgery M, Bispebjerg Hospital and Center for Healthy Aging, Faculty of Health Sciences, University of Copenhagen, Denmark

* Author to whom correspondence should be addressed.

Received: 13 November 2012; in revised form: 18 December 2012 / Accepted: 21 January 2013 / Published: 30 January 2013

(This article belongs to the Special Issue NMR-based Metabolomics and Its Application)

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Abstract: In the present study, proton NMR-based metabonomics was applied on femoral arterial plasma samples collected from young male subjects (milk protein n = 12 in a crossover design; non-caloric control n = 8) at different time intervals (70, 220, 370 min) after heavy resistance training and intake of either a whey or calcium caseinate protein drink in order to elucidate the impact of the protein source on post-exercise metabolism, which is important for muscle hypertrophy. Dynamic changes in the post-exercise plasma metabolite profile consisted of fluctuations in alanine, beta-hydroxybutyrate, branched amino acids, creatine, glucose, glutamine, glutamate, histidine, lipids and tyrosine. In comparison with the intake of a non-caloric drink, the same pattern of changes in low-molecular weight plasma metabolites was found for both whey and caseinate intake. However, the study indicated that whey and caseinate protein intake had a different impact on low-density and very-low-density lipoproteins present in the blood, which may be ascribed to different effects of the two protein sources on the mobilization of lipid resources during energy deficiency. In conclusion, no difference in the effects on low-molecular weight metabolites as measured by proton NMR-based metabonomics was found between the two protein sources.

Keywords: calcium caseinate; lipoproteins; metabonomics; protein metabolism; muscle hypertrophy; whey

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