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Metabolites 2012, 2(4), 959-982; doi:10.3390/metabo2040959
Article

Comparative Analysis of End Point Enzymatic Digests of Arabino-Xylan Isolated from Switchgrass (Panicum virgatum L) of Varying Maturities using LC-MSn

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Received: 14 September 2012; in revised form: 30 October 2012 / Accepted: 9 November 2012 / Published: 19 November 2012
(This article belongs to the Special Issue Glycomics and Glycoproteomics)
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Abstract: Switchgrass (Panicum virgatum L., SG) is a perennial grass presently used for forage and being developed as a bioenergy crop for conversion of cell wall carbohydrates to biofuels. Up to 50% of the cell wall associated carbohydrates are xylan. SG was analyzed for xylan structural features at variable harvest maturities. Xylan from each of three maturities was isolated using classical alkaline extraction to yield fractions (Xyl A and B) with varying compositional ratios. The Xyl B fraction was observed to decrease with plant age. Xylan samples were subsequently prepared for structure analysis by digesting with pure endo-xylanase, which preserved side-groups, or a commercial carbohydrase preparation favored for biomass conversion work. Enzymatic digestion products were successfully permethylated and analyzed by reverse-phase liquid chromatography with mass spectrometric detection (RP-HPLC-MSn). This method is advantageous compared to prior work on plant biomass because it avoids isolation of individual arabinoxylan oligomers. The use of RP-HPLC- MSn differentiated 14 structural oligosaccharides (d.p. 3–9) from the monocomponent enzyme digestion and nine oligosaccharide structures (d.p. 3–9) from hydrolysis with a cellulase enzyme cocktail. The distribution of arabinoxylan oligomers varied depending upon the enzyme(s) applied but did not vary with harvest maturity.
Keywords: switchgrass; xylan; xylooligosaccharides; LC-MSn; biofuel switchgrass; xylan; xylooligosaccharides; LC-MSn; biofuel
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Bowman, M.J.; Dien, B.S.; O'Bryan, P.J.; Sarath, G.; Cotta, M.A. Comparative Analysis of End Point Enzymatic Digests of Arabino-Xylan Isolated from Switchgrass (Panicum virgatum L) of Varying Maturities using LC-MSn. Metabolites 2012, 2, 959-982.

AMA Style

Bowman MJ, Dien BS, O'Bryan PJ, Sarath G, Cotta MA. Comparative Analysis of End Point Enzymatic Digests of Arabino-Xylan Isolated from Switchgrass (Panicum virgatum L) of Varying Maturities using LC-MSn. Metabolites. 2012; 2(4):959-982.

Chicago/Turabian Style

Bowman, Michael J.; Dien, Bruce S.; O'Bryan, Patricia J.; Sarath, Gautam; Cotta, Michael A. 2012. "Comparative Analysis of End Point Enzymatic Digests of Arabino-Xylan Isolated from Switchgrass (Panicum virgatum L) of Varying Maturities using LC-MSn." Metabolites 2, no. 4: 959-982.


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