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Separation Technique for the Determination of Highly Polar Metabolites in Biological Samples
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan
* Author to whom correspondence should be addressed.
Received: 8 June 2012; in revised form: 31 July 2012 / Accepted: 6 August 2012 / Published: 16 August 2012
Abstract: Metabolomics is a new approach that is based on the systematic study of the full complement of metabolites in a biological sample. Metabolomics has the potential to fundamentally change clinical chemistry and, by extension, the fields of nutrition, toxicology, and medicine. However, it can be difficult to separate highly polar compounds. Mass spectrometry (MS), in combination with capillary electrophoresis (CE), gas chromatography (GC), or high performance liquid chromatography (HPLC) is the key analytical technique on which emerging "omics" technologies, namely, proteomics, metabolomics, and lipidomics, are based. In this review, we introduce various methods for the separation of highly polar metabolites.
Keywords: capillary electrophoresis; gas chromatography; high performance liquid chromatography; highly polar metabolite
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Iwasaki, Y.; Sawada, T.; Hatayama, K.; Ohyagi, A.; Tsukuda, Y.; Namekawa, K.; Ito, R.; Saito, K.; Nakazawa, H. Separation Technique for the Determination of Highly Polar Metabolites in Biological Samples. Metabolites 2012, 2, 496-515.
Iwasaki Y, Sawada T, Hatayama K, Ohyagi A, Tsukuda Y, Namekawa K, Ito R, Saito K, Nakazawa H. Separation Technique for the Determination of Highly Polar Metabolites in Biological Samples. Metabolites. 2012; 2(3):496-515.
Iwasaki, Yusuke; Sawada, Takahiro; Hatayama, Kentaro; Ohyagi, Akihito; Tsukuda, Yuri; Namekawa, Kyohei; Ito, Rie; Saito, Koichi; Nakazawa, Hiroyuki. 2012. "Separation Technique for the Determination of Highly Polar Metabolites in Biological Samples." Metabolites 2, no. 3: 496-515.