Special Issue "Analytical Techniques in Metabolomics"
A special issue of Metabolites (ISSN 2218-1989).
Deadline for manuscript submissions: closed (30 April 2012)
Dr. Per Bruheim
Department of Biotechnology, Norwegian University of Science and Technology, 7034 Trondheim, Norway
Interests: mass spectrometry; metabolomics; metabolic engineering; secondary metabolites; biomakers
Metabolomics face particular analytical challenges due to the diverse physico-chemical properties of the metabolites; ranging from highly negatively charged organic acids and phosphometabolites to hydrophobic species such as fatty acids, steroids and pigments. Hence, at present no single analytical method can cover the whole Metabolome. Mass spectrometry (MS) and magnetic resonance (NMR) are the two main analytical technologies for analysis of metabolite pools, the former in combination with separation techniques as gas and liquid chromatography. Broadly, Metabolomics can be divided in two approaches: targeted and non-targeted analysis; the former delivering quantification of known metabolites while the latter approach is mostly used to classify samples and potential identification of unknown metabolites. Both approaches have benefitted strongly by recent technological developments, as the analytical instruments have become more sensitive and with higher resolution and accuracy. Therefore, this special issue in Metabolites will cover both qualitative and quantitative analytical techniques used to analyze metabolite pools as well as aspects on sample preparation and data processing.
Dr. Per Bruheim
Review: Targeted Chiral Analysis of Bioactive Arachidonic Acid Metabolites Using Liquid-Chromatography-Mass Spectrometry
Metabolites 2012, 2(2), 337-365; doi:10.3390/metabo2020337
Received: 1 March 2012; in revised form: 2 April 2012 / Accepted: 9 April 2012 / Published: 20 April 2012| Download PDF Full-text (943 KB) | Download XML Full-text
Article: An UPLC-ESI-MS/MS Assay Using 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate Derivatization for Targeted Amino Acid Analysis: Application to Screening of Arabidopsis thaliana Mutants
Metabolites 2012, 2(3), 398-428; doi:10.3390/metabo2030398
Received: 2 May 2012; in revised form: 29 June 2012 / Accepted: 4 July 2012 / Published: 6 July 2012| Download PDF Full-text (769 KB) | Download XML Full-text
Review: Separation Technique for the Determination of Highly Polar Metabolites in Biological Samples
Metabolites 2012, 2(3), 496-515; doi:10.3390/metabo2030496
Received: 8 June 2012; in revised form: 31 July 2012 / Accepted: 6 August 2012 / Published: 16 August 2012| Download PDF Full-text (300 KB) | Download XML Full-text
Review: A Guideline to Univariate Statistical Analysis for LC/MS-Based Untargeted Metabolomics-Derived Data
Metabolites 2012, 2(4), 775-795; doi:10.3390/metabo2040775
Received: 2 August 2012; in revised form: 2 October 2012 / Accepted: 10 October 2012 / Published: 18 October 2012| Download PDF Full-text (420 KB) | Download XML Full-text
Technical Note: A Comprehensive Workflow of Mass Spectrometry-Based Untargeted Metabolomics in Cancer Metabolic Biomarker Discovery Using Human Plasma and Urine
Metabolites 2013, 3(3), 787-819; doi:10.3390/metabo3030787
Received: 10 July 2013; in revised form: 30 August 2013 / Accepted: 2 September 2013 / Published: 11 September 2013| Download PDF Full-text (1870 KB) | Download XML Full-text
Last update: 27 February 2014