Special Issue "Analytical Techniques in Metabolomics"
A special issue of Metabolites (ISSN 2218-1989).
Deadline for manuscript submissions: closed (30 April 2012)
Dr. Per Bruheim
Department of Biotechnology, Norwegian University of Science and Technology, 7034 Trondheim, Norway
Interests: mass spectrometry; metabolomics; metabolic engineering; secondary metabolites; biomakers
Metabolomics face particular analytical challenges due to the diverse physico-chemical properties of the metabolites; ranging from highly negatively charged organic acids and phosphometabolites to hydrophobic species such as fatty acids, steroids and pigments. Hence, at present no single analytical method can cover the whole Metabolome. Mass spectrometry (MS) and magnetic resonance (NMR) are the two main analytical technologies for analysis of metabolite pools, the former in combination with separation techniques as gas and liquid chromatography. Broadly, Metabolomics can be divided in two approaches: targeted and non-targeted analysis; the former delivering quantification of known metabolites while the latter approach is mostly used to classify samples and potential identification of unknown metabolites. Both approaches have benefitted strongly by recent technological developments, as the analytical instruments have become more sensitive and with higher resolution and accuracy. Therefore, this special issue in Metabolites will cover both qualitative and quantitative analytical techniques used to analyze metabolite pools as well as aspects on sample preparation and data processing.
Dr. Per Bruheim
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. Papers will be published continuously (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are refereed through a peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Metabolites is an international peer-reviewed Open Access quarterly journal published by MDPI.
Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 300 CHF (Swiss Francs). English correction and/or formatting fees of 250 CHF (Swiss Francs) will be charged in certain cases for those articles accepted for publication that require extensive additional formatting and/or English corrections.
Review: Targeted Chiral Analysis of Bioactive Arachidonic Acid Metabolites Using Liquid-Chromatography-Mass Spectrometry
Metabolites 2012, 2(2), 337-365; doi:10.3390/metabo2020337
Received: 1 March 2012; in revised form: 2 April 2012 / Accepted: 9 April 2012 / Published: 20 April 2012| Download PDF Full-text (943 KB) | Download XML Full-text
Article: An UPLC-ESI-MS/MS Assay Using 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate Derivatization for Targeted Amino Acid Analysis: Application to Screening of Arabidopsis thaliana Mutants
Metabolites 2012, 2(3), 398-428; doi:10.3390/metabo2030398
Received: 2 May 2012; in revised form: 29 June 2012 / Accepted: 4 July 2012 / Published: 6 July 2012| Download PDF Full-text (769 KB) | Download XML Full-text
Review: Separation Technique for the Determination of Highly Polar Metabolites in Biological Samples
Metabolites 2012, 2(3), 496-515; doi:10.3390/metabo2030496
Received: 8 June 2012; in revised form: 31 July 2012 / Accepted: 6 August 2012 / Published: 16 August 2012| Download PDF Full-text (300 KB) | Download XML Full-text
Review: A Guideline to Univariate Statistical Analysis for LC/MS-Based Untargeted Metabolomics-Derived Data
Metabolites 2012, 2(4), 775-795; doi:10.3390/metabo2040775
Received: 2 August 2012; in revised form: 2 October 2012 / Accepted: 10 October 2012 / Published: 18 October 2012| Download PDF Full-text (420 KB) | Download XML Full-text
Technical Note: A Comprehensive Workflow of Mass Spectrometry-Based Untargeted Metabolomics in Cancer Metabolic Biomarker Discovery Using Human Plasma and Urine
Metabolites 2013, 3(3), 787-819; doi:10.3390/metabo3030787
Received: 10 July 2013; in revised form: 30 August 2013 / Accepted: 2 September 2013 / Published: 11 September 2013| Download PDF Full-text (1870 KB) | Download XML Full-text
The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.
Type of Paper: Article
Title: The Comparison of Lipid Extraction Methods for the "Lipidomic" Analysis of Blood Plasma and Brown Adipose Tissue by H1 Nuclear Magnetic Resonance Spectroscopy
Author: Clare A. Daykin
Affiliation: Analytical Biosciences, Division of Molecular and Cellular Science, Centre for Biomolecular Sciences, School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, UK; E-mail: firstname.lastname@example.org
Abstract: Whilst lipids can be studied in whole biosamples (whether fluids or tissues) using various NMR spectroscopy-based spectral editing methods without sample extraction, if these methods are used in isolation, valuable information may be lost. Hence, the evaluation of methods which will allow efficient and reproducible extraction of lipids from biosamples is critical. In the literature, many different protocols for lipid extraction are reported, however; there is a severe lack of data comparing their efficacy and reproducibility. This study has therefore investigated the variations among different methods by extracting lipids from blood plasma and brown adipose tissue. The results presented here show that these lipid extraction methods vary considerably in the recovery of lipids and a judicious choice is crucial for optimal extraction. Whilst the chloroform/methanol based Bligh and Dyer extraction method appears to be the most reproducible method, it requires large amounts of sample and is known to underestimate lipid concentrations in samples where lipids constitute > 2% of the sample. On the other hand, the Folch method allows the detection of more lipids and at higher concentrations than other methods whilst retaining the advantage of relatively good reproducibility.
Last update: 12 October 2012