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Comparative Lipidomic Profiling of S. cerevisiae and Four Other Hemiascomycetous Yeasts
Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V., Otto-Hahn-Str. 6b, D-44227 Dortmund, Germany
Current address: Bayer CropScience AG, BCS AG-D-EnSa-EFate (Environmental Fate), Alfred-Nobel-Str. 50, D-40789 Monheim am Rhein, Germany.
Current address: Department of Food Chemistry, University of Wuppertal, Gaußstr. 20, D-42119 Wuppertal, Germany.
* Author to whom correspondence should be addressed.
Received: 19 January 2012; in revised form: 23 February 2012 / Accepted: 24 February 2012 / Published: 2 March 2012
(This article belongs to the Special Issue Lipidomics
Abstract: Glycerophospholipids (GP) are the building blocks of cellular membranes and play essential roles in cell compartmentation, membrane fluidity or apoptosis. In addition, GPs are sources for multifunctional second messengers. Whereas the genome and proteome of the most intensively studied eukaryotic model organism, the baker’s yeast (Saccharomyces cerevisiae), are well characterized, the analysis of its lipid composition is still at the beginning. Moreover, different yeast species can be distinguished on the DNA, RNA and protein level, but it is currently unknown if they can also be differentiated by determination of their GP pattern. Therefore, the GP compositions of five different yeast strains, grown under identical environmental conditions, were elucidated using high performance liquid chromatography coupled to negative electrospray ionization-hybrid linear ion trap-Fourier transform ion cyclotron resonance mass spectrometry in single and multistage mode. Using this approach, relative quantification of more than 100 molecular species belonging to nine GP classes was achieved. The comparative lipidomic profiling of Saccharomyces cerevisiae, Saccharomyces bayanus, Kluyveromyces thermotolerans, Pichia angusta, and Yarrowia lipolytica revealed characteristic GP profiles for each strain. However, genetically related yeast strains show similarities in their GP compositions, e.g., Saccharomyces cerevisiae and Saccharomyces bayanus.
Keywords: yeast strains; Saccharomyces cerevisiae; Saccharomyces bayanus; Kluyveromyces thermotolerans; Pichia angusta; Yarrowia lipolytica; glycerophospholipid profiling; LC-MS; lipidomics
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Hein, E.-M.; Hayen, H. Comparative Lipidomic Profiling of S. cerevisiae and Four Other Hemiascomycetous Yeasts. Metabolites 2012, 2, 254-267.
Hein E-M, Hayen H. Comparative Lipidomic Profiling of S. cerevisiae and Four Other Hemiascomycetous Yeasts. Metabolites. 2012; 2(1):254-267.
Hein, Eva-Maria; Hayen, Heiko. 2012. "Comparative Lipidomic Profiling of S. cerevisiae and Four Other Hemiascomycetous Yeasts." Metabolites 2, no. 1: 254-267.