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Metabolites 2012, 2(1), 254-267; doi:10.3390/metabo2010254
Article

Comparative Lipidomic Profiling of S. cerevisiae and Four Other Hemiascomycetous Yeasts

 and ‡,*
Received: 19 January 2012; in revised form: 23 February 2012 / Accepted: 24 February 2012 / Published: 2 March 2012
(This article belongs to the Special Issue Lipidomics)
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Abstract: Glycerophospholipids (GP) are the building blocks of cellular membranes and play essential roles in cell compartmentation, membrane fluidity or apoptosis. In addition, GPs are sources for multifunctional second messengers. Whereas the genome and proteome of the most intensively studied eukaryotic model organism, the baker’s yeast (Saccharomyces cerevisiae), are well characterized, the analysis of its lipid composition is still at the beginning. Moreover, different yeast species can be distinguished on the DNA, RNA and protein level, but it is currently unknown if they can also be differentiated by determination of their GP pattern. Therefore, the GP compositions of five different yeast strains, grown under identical environmental conditions, were elucidated using high performance liquid chromatography coupled to negative electrospray ionization-hybrid linear ion trap-Fourier transform ion cyclotron resonance mass spectrometry in single and multistage mode. Using this approach, relative quantification of more than 100 molecular species belonging to nine GP classes was achieved. The comparative lipidomic profiling of Saccharomyces cerevisiae, Saccharomyces bayanus, Kluyveromyces thermotolerans, Pichia angusta, and Yarrowia lipolytica revealed characteristic GP profiles for each strain. However, genetically related yeast strains show similarities in their GP compositions, e.g., Saccharomyces cerevisiae and Saccharomyces bayanus.
Keywords: yeast strains; Saccharomyces cerevisiae; Saccharomyces bayanus; Kluyveromyces thermotolerans; Pichia angusta; Yarrowia lipolytica; glycerophospholipid profiling; LC-MS; lipidomics yeast strains; Saccharomyces cerevisiae; Saccharomyces bayanus; Kluyveromyces thermotolerans; Pichia angusta; Yarrowia lipolytica; glycerophospholipid profiling; LC-MS; lipidomics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Hein, E.-M.; Hayen, H. Comparative Lipidomic Profiling of S. cerevisiae and Four Other Hemiascomycetous Yeasts. Metabolites 2012, 2, 254-267.

AMA Style

Hein E-M, Hayen H. Comparative Lipidomic Profiling of S. cerevisiae and Four Other Hemiascomycetous Yeasts. Metabolites. 2012; 2(1):254-267.

Chicago/Turabian Style

Hein, Eva-Maria; Hayen, Heiko. 2012. "Comparative Lipidomic Profiling of S. cerevisiae and Four Other Hemiascomycetous Yeasts." Metabolites 2, no. 1: 254-267.


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