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Sci. Pharm. 2011, 79(4), 951-962; doi:10.3797/scipharm.1101-15

Induction of Biologically Active Flavonoids in Cell Cultures of Morus nigra and Testing their Hypoglycemic Efficacy

1
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt
2
Department of Pharmacognosy, Faculty of Pharmacy, Taif University, Taif 21974, Saudi Arabia
3
Department of Anatomy and Histology, Faculty of Medicine and Medical sciences, Taif University, Taif 21974, Saudi Arabia
*
Author to whom correspondence should be addressed.
Received: 22 January 2011 / Accepted: 3 October 2011 / Published: 3 October 2011
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Abstract

The antidiabetic activity of both leaves and MJ-treated cell cultures of Morus nigra was evaluated after their oral administration to streptozotocin-induced diabetic rats. The antidiabetic activity of extracts from leaves given to streptozotocin (STZ)-diabetic rats for 10 days increased with increasing doses of leaves extract up to 500 mg/kg/day. The administration of 500 mg/kg/day of leaves extract reduced the concentration of glucose from 370 ± 7.31 mg/dl (control) to 154 ± 6.27 mg/dl, and a significant increase in the insulin level from 11.3 ± 0.31 μU/ml (control) to 14.6 ± 0.43 μU/ml was recorded. Cell suspension cultures were established from the young leaves of Morus nigra cultivated on modified MS medium supplemented with 2.0 mg/l 1-naphthaleneacetic acid (NAA), 0.2 mg/l 6-(furfurylamino)purine (kinetin). The changes in cell weight and flavonoid content were monitored between day zero and 12. The linear increase in fresh weight was found to be parallel to flavonoids production. Cell cultures treated with 100 μM methyl jasmonate for 24 hours showed a noticeable increase in level of flavonoids and significant and more effective hypoglycemic activity than that for extract from leaves. The major flavonoids were isolated by TLC and HPLC and identified as rutin, quercetin, Morusin and cyclomorusin by co-chromatography and mass spectrometry in comparison to samples of authentic reference compounds.
Keywords: Morus nigra; MS medium; Methyl jasmonate; Flavonoids; Streptozotocin Morus nigra; MS medium; Methyl jasmonate; Flavonoids; Streptozotocin
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

ABD EL-MAWLA, A.M.A.; MOHAMED, K.M.; MOSTAFA, A.M. Induction of Biologically Active Flavonoids in Cell Cultures of Morus nigra and Testing their Hypoglycemic Efficacy. Sci. Pharm. 2011, 79, 951-962.

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