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Biology 2017, 6(4), 37; doi:10.3390/biology6040037

Artificial RNA Motifs Expand the Programmable Assembly between RNA Modules of a Bimolecular Ribozyme Leading to Application to RNA Nanostructure Design

1
Department of Chemistry, Graduate School of Science and Engineering, University of Toyama, Gofuku 3190, Toyama 9308555, Japan
2
Graduate School of Innovative Life Science, University of Toyama, Gofuku 3190, Toyama 9308555, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Jukka Finne
Received: 17 September 2017 / Revised: 21 October 2017 / Accepted: 25 October 2017 / Published: 30 October 2017
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Abstract

A bimolecular ribozyme consisting of a core ribozyme (ΔP5 RNA) and an activator module (P5abc RNA) has been used as a platform to design assembled RNA nanostructures. The tight and specific assembly between the P5abc and ΔP5 modules depends on two sets of intermodule interactions. The interface between P5abc and ΔP5 must be controlled when designing RNA nanostructures. To expand the repertoire of molecular recognition in the P5abc/ΔP5 interface, we modified the interface by replacing the parent tertiary interactions in the interface with artificial interactions. The engineered P5abc/ΔP5 interfaces were characterized biochemically to identify those suitable for nanostructure design. The new interfaces were used to construct 2D-square and 1D-array RNA nanostructures. View Full-Text
Keywords: catalytic RNA; group I intron; ribozyme; RNA nanostructure; RNA nanotechnology catalytic RNA; group I intron; ribozyme; RNA nanostructure; RNA nanotechnology
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Rahman, M.M.; Matsumura, S.; Ikawa, Y. Artificial RNA Motifs Expand the Programmable Assembly between RNA Modules of a Bimolecular Ribozyme Leading to Application to RNA Nanostructure Design. Biology 2017, 6, 37.

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