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Biology 2017, 6(1), 9; doi:10.3390/biology6010009

Quantitative Profiling of Hydroxy Lipid Metabolites in Mouse Organs Reveals Distinct Lipidomic Profiles and Modifications Due to Elevated n-3 Fatty Acid Levels

1
Department of Medicine, Division of Hepatology and Gastroenterology, Charité University Medicine Berlin, Campus Virchow-Klinikum, 13353 Berlin, Germany
2
Lipid Clinic, Experimental and Clinical Research Centre (ECRC), Charité University Medicine and Max-Delbrück-Center for Molecular Medicine, 13125 Berlin, Germany
3
Lipidomix, 13125 Berlin, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Chris O’Callaghan
Received: 21 November 2016 / Revised: 20 January 2017 / Accepted: 22 January 2017 / Published: 4 February 2017
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Abstract

Polyunsaturated fatty acids (PUFA) are precursors of bioactive metabolites and mediators. In this study, the profile of hydroxyeicosatetraenoic (HETE), hydroxyeicosapentaenoic (HEPE) and hydroxydocosahexaenoic (HDHA) acids derived from arachidonic acid (AA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in colon, liver, lung, spleen, muscle, heart and kidney tissue of healthy wildtype mice were characterized, and compared to profiles in organs from transgenic fat-1 mice engineered to express the Caenorhabditis elegans fat-1 gene encoding an n-3 desaturase and thereby with endogenously elevated n-3 PUFA levels. PUFAs were measured using gas chromatography. The lipid metabolites were assayed using LC-MS/MS. AA and DHA were the prominent PUFAs in wildtype and fat-1 mice. EPA levels were low in both groups even though there was a significant increase in fat-1 organs with an up to 12-fold increase in fat-1 spleen and kidney. DHA levels increased by approximately 1.5-fold in fat-1 as compared to wildtype mice. While HETEs remained the same or decreased moderately and HDHAs increased 1- to 3-fold, HEPE formation in fat-1 tissues increased from 8- (muscle) to 44-fold (spleen). These findings indicate distinct profiles of monohydroxy lipid metabolites in different organs and strong utilization of EPA for HEPE formation, by which moderate EPA supplementation might trigger formation of biologically active EPA-derived resolvins. View Full-Text
Keywords: arachidonic acid; eicosapentaenoic acid; docosahexaenoic acid; HETE; HEPE; HDHA; fat-1; polyunsaturated fatty acids; omega-6; omega-3 arachidonic acid; eicosapentaenoic acid; docosahexaenoic acid; HETE; HEPE; HDHA; fat-1; polyunsaturated fatty acids; omega-6; omega-3
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Chiu, C.-Y.; Smyl, C.; Dogan, I.; Rothe, M.; Weylandt, K.-H. Quantitative Profiling of Hydroxy Lipid Metabolites in Mouse Organs Reveals Distinct Lipidomic Profiles and Modifications Due to Elevated n-3 Fatty Acid Levels. Biology 2017, 6, 9.

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