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Biology 2014, 3(1), 167-175; doi:10.3390/biology3010167

In Vitro High Throughput Screening, What Next? Lessons from the Screening for Aurora Kinase Inhibitors

1 INSERM UJF U823 Institut Albert Bonniot, Team 4: Chromatin and Epigenetic, BP 170, 38 042 Grenoble Cedex 9, France 2 Faculty of Biology, VNU University of Science, HaNoi 10 000, Vietnam 3 Department of biology, University of Natural Sciences, Ho Chi Minh 70 000, Vietnam 4 Department of biology, University of Technology, DaNang 55 000, Vietnam 5 UMR 176 CNRS-Institut Curie, Bat 110 Centre Universitaire, Orsay 91405, France
* Author to whom correspondence should be addressed.
Received: 3 December 2013 / Revised: 13 February 2014 / Accepted: 14 February 2014 / Published: 27 February 2014
(This article belongs to the Special Issue Screening for Biologically Active Compounds)
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Based on in vitro assays, we performed a High Throughput Screening (HTS) to identify kinase inhibitors among 10,000 small chemical compounds. In this didactic paper, we describe step-by-step the approach to validate the hits as well as the major pitfalls encountered in the development of active molecules. We propose a decision tree that could be adapted to most in vitro HTS.
Keywords: aurora; kinase; kinase inhibitor; Gck; Nuak1; Tak-1 aurora; kinase; kinase inhibitor; Gck; Nuak1; Tak-1
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Hoang, T.-M.-N.; Vu, H.-L.; Le, L.-T.-T.; Nguyen, C.-H.; Molla, A. In Vitro High Throughput Screening, What Next? Lessons from the Screening for Aurora Kinase Inhibitors. Biology 2014, 3, 167-175.

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