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Biology 2014, 3(1), 167-175; doi:10.3390/biology3010167

In Vitro High Throughput Screening, What Next? Lessons from the Screening for Aurora Kinase Inhibitors

INSERM UJF U823 Institut Albert Bonniot, Team 4: Chromatin and Epigenetic, BP 170, 38 042 Grenoble Cedex 9, France
Faculty of Biology, VNU University of Science, HaNoi 10 000, Vietnam
Department of biology, University of Natural Sciences, Ho Chi Minh 70 000, Vietnam
Department of biology, University of Technology, DaNang 55 000, Vietnam
UMR 176 CNRS-Institut Curie, Bat 110 Centre Universitaire, Orsay 91405, France
Author to whom correspondence should be addressed.
Received: 3 December 2013 / Revised: 13 February 2014 / Accepted: 14 February 2014 / Published: 27 February 2014
(This article belongs to the Special Issue Screening for Biologically Active Compounds)
View Full-Text   |   Download PDF [387 KB, uploaded 27 February 2014]   |  


Based on in vitro assays, we performed a High Throughput Screening (HTS) to identify kinase inhibitors among 10,000 small chemical compounds. In this didactic paper, we describe step-by-step the approach to validate the hits as well as the major pitfalls encountered in the development of active molecules. We propose a decision tree that could be adapted to most in vitro HTS.
Keywords: aurora; kinase; kinase inhibitor; Gck; Nuak1; Tak-1 aurora; kinase; kinase inhibitor; Gck; Nuak1; Tak-1

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Hoang, T.-M.-N.; Vu, H.-L.; Le, L.-T.-T.; Nguyen, C.-H.; Molla, A. In Vitro High Throughput Screening, What Next? Lessons from the Screening for Aurora Kinase Inhibitors. Biology 2014, 3, 167-175.

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