Biology 2014, 3(1), 167-175; doi:10.3390/biology3010167
Article

In Vitro High Throughput Screening, What Next? Lessons from the Screening for Aurora Kinase Inhibitors

1,2email, 1,3email, 1,4email, 5email and 1,* email
Received: 3 December 2013; in revised form: 13 February 2014 / Accepted: 14 February 2014 / Published: 27 February 2014
(This article belongs to the Special Issue Screening for Biologically Active Compounds)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Based on in vitro assays, we performed a High Throughput Screening (HTS) to identify kinase inhibitors among 10,000 small chemical compounds. In this didactic paper, we describe step-by-step the approach to validate the hits as well as the major pitfalls encountered in the development of active molecules. We propose a decision tree that could be adapted to most in vitro HTS.
Keywords: aurora; kinase; kinase inhibitor; Gck; Nuak1; Tak-1
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MDPI and ACS Style

Hoang, T.-M.-N.; Vu, H.-L.; Le, L.-T.-T.; Nguyen, C.-H.; Molla, A. In Vitro High Throughput Screening, What Next? Lessons from the Screening for Aurora Kinase Inhibitors. Biology 2014, 3, 167-175.

AMA Style

Hoang T-M-N, Vu H-L, Le L-T-T, Nguyen C-H, Molla A. In Vitro High Throughput Screening, What Next? Lessons from the Screening for Aurora Kinase Inhibitors. Biology. 2014; 3(1):167-175.

Chicago/Turabian Style

Hoang, Thi-My-Nhung; Vu, Hong-Lien; Le, Ly-Thuy-Tram; Nguyen, Chi-Hung; Molla, Annie. 2014. "In Vitro High Throughput Screening, What Next? Lessons from the Screening for Aurora Kinase Inhibitors." Biology 3, no. 1: 167-175.

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