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Genotyping-by-Sequencing in Plants
DuPont Agricultural Biotechnology, Experimental Station, PO Box 80353, 200 Powder Mill Road, Wilmington, DE 19880-0353, USA
DuPont Pioneer, 7300 NW 62nd Ave., P.O. Box 1004, Johnston, IA 50131-1004, USA
* Author to whom correspondence should be addressed.
Received: 6 August 2012; in revised form: 7 August 2012 / Accepted: 13 September 2012 / Published: 25 September 2012
Abstract: The advent of next-generation DNA sequencing (NGS) technologies has led to the development of rapid genome-wide Single Nucleotide Polymorphism (SNP) detection applications in various plant species. Recent improvements in sequencing throughput combined with an overall decrease in costs per gigabase of sequence is allowing NGS to be applied to not only the evaluation of small subsets of parental inbred lines, but also the mapping and characterization of traits of interest in much larger populations. Such an approach, where sequences are used simultaneously to detect and score SNPs, therefore bypassing the entire marker assay development stage, is known as genotyping-by-sequencing (GBS). This review will summarize the current state of GBS in plants and the promises it holds as a genome-wide genotyping application.
Keywords: genotyping; genotyping-by-sequencing; sequencing; next-generation sequencing; genome-wide association; DNA; SNPs; NGS; GBS
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Deschamps, S.; Llaca, V.; May, G.D. Genotyping-by-Sequencing in Plants. Biology 2012, 1, 460-483.
Deschamps S, Llaca V, May GD. Genotyping-by-Sequencing in Plants. Biology. 2012; 1(3):460-483.
Deschamps, Stéphane; Llaca, Victor; May, Gregory D. 2012. "Genotyping-by-Sequencing in Plants." Biology 1, no. 3: 460-483.