Biology 2012, 1(3), 484-494; doi:10.3390/biology1030484
Article

Bioinformatics Analysis of the FREM1 Gene—Evolutionary Development of the IL-1R1 Co-Receptor, TILRR

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Received: 23 July 2012; in revised form: 28 August 2012 / Accepted: 3 September 2012 / Published: 25 September 2012
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The TLRs and IL-1 receptors have evolved to coordinate the innate immune response following pathogen invasion. Receptors and signalling intermediates of these systems are generally characterised by a high level of evolutionary conservation. The recently described IL-1R1 co-receptor TILRR is a transcriptional variant of the FREM1 gene. Here we investigate whether innate co-receptor differences between teleosts and mammals extend to the expression of the TILRR isoform of FREM1. Bioinformatic and phylogenetic approaches were used to analyse the genome sequences of FREM1 from eukaryotic organisms including 37 tetrapods and five teleost fish. The TILRR consensus peptide sequence was present in the FREM1 gene of the tetrapods, but not in fish orthologs of FREM1, and neither FREM1 nor TILRR were present in invertebrates. The TILRR gene appears to have arisen via incorporation of adjacent non-coding DNA with a contiguous exonic sequence after the teleost divergence. Comparing co-receptors in other systems, points to their origin during the same stages of evolution. Our results show that modern teleost fish do not possess the IL-1RI co-receptor TILRR, but that this is maintained in tetrapods as early as amphibians. Further, they are consistent with data showing that co-receptors are recent additions to these regulatory systems and suggest this may underlie differences in innate immune responses between mammals and fish.
Keywords: TILRR; IL-1RI; co-receptor; FREM1; bioinformatics; evolutionary development; signal transduction; TIR activation; IL-1
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MDPI and ACS Style

Hudson, R.C.; Gray, C.; Kiss-Toth, E.; Chico, T.J.A.; Qwarnstrom, E.E. Bioinformatics Analysis of the FREM1 Gene—Evolutionary Development of the IL-1R1 Co-Receptor, TILRR. Biology 2012, 1, 484-494.

AMA Style

Hudson RC, Gray C, Kiss-Toth E, Chico TJA, Qwarnstrom EE. Bioinformatics Analysis of the FREM1 Gene—Evolutionary Development of the IL-1R1 Co-Receptor, TILRR. Biology. 2012; 1(3):484-494.

Chicago/Turabian Style

Hudson, Richard C.; Gray, Caroline; Kiss-Toth, Endre; Chico, Timothy J. A.; Qwarnstrom, Eva E. 2012. "Bioinformatics Analysis of the FREM1 Gene—Evolutionary Development of the IL-1R1 Co-Receptor, TILRR." Biology 1, no. 3: 484-494.


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