Biology 2012, 1(2), 383-394; doi:10.3390/biology1020383

Next-Generation Sequencing: Application in Liver Cancer—Past, Present and Future?

1,2,* email and 2,* email
Received: 25 July 2012; in revised form: 14 August 2012 / Accepted: 20 August 2012 / Published: 31 August 2012
(This article belongs to the Special Issue Next Generation Sequencing Approaches in Biology)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Hepatocellular Carcinoma (HCC) is the third most deadly malignancy worldwide characterized by phenotypic and molecular heterogeneity. In the past two decades, advances in genomic analyses have formed a comprehensive understanding of different underlying pathobiological layers resulting in hepatocarcinogenesis. More recently, improvements of sophisticated next-generation sequencing (NGS) technologies have enabled complete and cost-efficient analyses of cancer genomes at a single nucleotide resolution and advanced into valuable tools in translational medicine. Although the use of NGS in human liver cancer is still in its infancy, great promise rests in the systematic integration of different molecular analyses obtained by these methodologies, i.e., genomics, transcriptomics and epigenomics. This strategy is likely to be helpful in identifying relevant and recurrent pathophysiological hallmarks thereby elucidating our limited understanding of liver cancer. Beside tumor heterogeneity, progress in translational oncology is challenged by the amount of biological information and considerable “noise” in the data obtained from different NGS platforms. Nevertheless, the following review aims to provide an overview of the current status of next-generation approaches in liver cancer, and outline the prospects of these technologies in diagnosis, patient classification, and prediction of outcome. Further, the potential of NGS to identify novel applications for concept clinical trials and to accelerate the development of new cancer therapies will be summarized.
Keywords: Hepatocellular carcinoma (HCC); Next-generation sequencing (NGS); personalized medicine; integrative genomics
PDF Full-text Download PDF Full-Text [143 KB, uploaded 31 August 2012 12:06 CEST]

Export to BibTeX |

MDPI and ACS Style

Marquardt, J.U.; Andersen, J.B. Next-Generation Sequencing: Application in Liver Cancer—Past, Present and Future? Biology 2012, 1, 383-394.

AMA Style

Marquardt JU, Andersen JB. Next-Generation Sequencing: Application in Liver Cancer—Past, Present and Future? Biology. 2012; 1(2):383-394.

Chicago/Turabian Style

Marquardt, Jens U.; Andersen, Jesper B. 2012. "Next-Generation Sequencing: Application in Liver Cancer—Past, Present and Future?" Biology 1, no. 2: 383-394.

Biology EISSN 2079-7737 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert