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Fibers 2014, 2(4), 285-294; doi:10.3390/fib2040285

Characterization of Developing Cotton Fibers by Confocal Raman Microscopy

Department of Physics and Engineering, California State University at Bakersfield, 9001 Stockdale Highway, Bakersfield, CA 93311, USA
Fiber and Biopolymer Research Institute, Department of Plant and Soil Science, Texas Tech University, Lubbock, TX 79403, USA
Department of Physics, University of Texas at El Paso, El Paso, TX 79968, USA
Author to whom correspondence should be addressed.
Received: 21 September 2014 / Revised: 17 October 2014 / Accepted: 22 October 2014 / Published: 27 October 2014
(This article belongs to the Special Issue Cellulose Fibers)
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Cellulose deposition in developing cotton fibers has been studied previously with analytical techniques, such as Fourier transform infrared spectroscopy (FTIR), High-performance liquid chromatography (HPLC) and Thermogravimetric analysis (TGA). Recent technological developments in instrumentation have made Raman microscopy emerge as an extraordinary analytical tool in biological and plant research. The advantage of using confocal Raman microscopy (CRM) resides in the lateral spatial resolution and in the fact that Raman spectroscopy provides not only chemical composition information, but also structural information. Cross-sections of cotton fibers harvested at different developmental stages were studied with CRM. The Raman bands assigned to cellulose were analyzed. The results of this study indicate that CRM can be used as a tool to study cellulose deposition in cotton fibers and could provide useful information on cellulose deposition during cotton fiber development. View Full-Text
Keywords: cotton; confocal Raman microscopy; X-Ray Diffraction (XRD); cellulose crystallinity; fiber development; label-free imaging cotton; confocal Raman microscopy; X-Ray Diffraction (XRD); cellulose crystallinity; fiber development; label-free imaging

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Cabrales, L.; Abidi, N.; Manciu, F. Characterization of Developing Cotton Fibers by Confocal Raman Microscopy. Fibers 2014, 2, 285-294.

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