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Coatings 2016, 6(1), 9; doi:10.3390/coatings6010009

Growth and Functionality of Cells Cultured on Conducting and Semi-Conducting Surfaces Modified with Self-Assembled Monolayers (SAMs)

1
Institute of Micromanufacturing, Louisiana Tech University, Ruston, LA 71272, USA
2
School of Biological Sciences, Louisiana Tech University, Ruston, LA 71272, USA
3
Center for Biomedical Engineering and Rehabilitation Science, Louisiana Tech University, Ruston, LA 71272, USA
4
Department of Chemistry, Department of Nanoengineering, Joint School of Nanoscience and Nanoengineering, North Carolina A & T State University, Greensboro, NC 27411, USA
*
Author to whom correspondence should be addressed.
Academic Editor: James Kit-hon Tsoi
Received: 22 November 2015 / Revised: 23 January 2016 / Accepted: 28 January 2016 / Published: 18 February 2016
View Full-Text   |   Download PDF [3167 KB, uploaded 18 February 2016]   |  

Abstract

Bioengineering of dermal and epidermal cells on surface modified substrates is an active area of research. The cytotoxicity, maintenance of cell phenotype and long-term functionality of human dermal fibroblast (HDF) cells on conducting indium tin oxide (ITO) and semi-conducting, silicon (Si) and gallium arsenide (GaAs), surfaces modified with self-assembled monolayers (SAMs) containing amino (–NH2) and methyl (–CH3) end groups have been investigated. Contact angle measurements and infrared spectroscopic studies show that the monolayers are conformal and preserve their functional end groups. Morphological analyses indicate that HDFs grow well on all substrates except GaAs, exhibiting their normal spindle-shaped morphology and exhibit no visible signs of stress or cytoplasmic vacuolation. Cell viability analyses indicate little cell death after one week in culture on all substrates except GaAs, where cells died within 6 h. Cells on all surfaces proliferate except on GaAs and GaAs-ODT. Cell growth is observed to be greater on SAM modified ITO and Si-substrates. Preservation of cellular phenotype assessed through type I collagen immunostaining and positive staining of HDF cells were observed on all modified surfaces except that on GaAs. These results suggest that conducting and semi-conducting SAM-modified surfaces support HDF growth and functionality and represent a promising area of bioengineering research. View Full-Text
Keywords: human dermal fibroblasts; self-assembled monolayer; amino group; proliferation; GaAs; ITO; conducting surface human dermal fibroblasts; self-assembled monolayer; amino group; proliferation; GaAs; ITO; conducting surface
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Aithal, R.K.; Doss, A.T.; Kumaraswamy, D.P.; Mills, D.K.; Kuila, D. Growth and Functionality of Cells Cultured on Conducting and Semi-Conducting Surfaces Modified with Self-Assembled Monolayers (SAMs). Coatings 2016, 6, 9.

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