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Coatings 2014, 4(3), 450-464; doi:10.3390/coatings4030450

The GM1 Ganglioside Forms GM1-Rich Gel Phase Microdomains within Lipid Rafts

1
Istituto di Chimica dei Composti Organo Metallici, Consiglio Nazionale delle Ricerche, Via Madonna del Piano 10, 50019 Sesto Fiorentino (Firenze), Italy
2
Department of Chemistry, Florence University, Sesto Fiorentino (Firenze) 50019, Italy
Retired.
*
Author to whom correspondence should be addressed.
Received: 30 May 2014 / Revised: 1 July 2014 / Accepted: 7 July 2014 / Published: 16 July 2014
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Abstract

Mercury-supported, self-assembled monolayers (SAMs) of the sole dioleoylphosphatidylcholine (DOPC) and of a raft-forming mixture of DOPC, cholesterol (Chol) and palmitoylsphingomyelin (PSM) of (59:26:15) mol% composition, were investigated by electrochemical impedance spectroscopy (EIS), both in the absence and in the presence of the monosialoganglioside GM1. The impedance spectra of these four SAMs were fitted by a series of parallel combinations of a resistance and a capacitance (RC meshes) and displayed on plots of ωZ′ against −ωZ″, where Z′ and Z″ are the in-phase and quadrature components of the impedance and ω is the angular frequency. A comparison among these different impedance spectra points to the formation of GM1-rich gel phase microdomains within the lipid rafts of the DOPC/Chol/PSM mixture, thanks to the unique molecular-level smooth support provided by mercury, which allows EIS to detect the protruding gel phase microdomains by averaging them over a macroscopically large area. View Full-Text
Keywords: lipid rafts; gel phase microdomains; electrochemical impedance spectroscopy; cholera toxin lipid rafts; gel phase microdomains; electrochemical impedance spectroscopy; cholera toxin
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Becucci, L.; Vizza, F.; Duarte, Y.; Guidelli, R. The GM1 Ganglioside Forms GM1-Rich Gel Phase Microdomains within Lipid Rafts. Coatings 2014, 4, 450-464.

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