The GM1 Ganglioside Forms GM1-Rich Gel Phase Microdomains within Lipid Rafts
AbstractMercury-supported, self-assembled monolayers (SAMs) of the sole dioleoylphosphatidylcholine (DOPC) and of a raft-forming mixture of DOPC, cholesterol (Chol) and palmitoylsphingomyelin (PSM) of (59:26:15) mol% composition, were investigated by electrochemical impedance spectroscopy (EIS), both in the absence and in the presence of the monosialoganglioside GM1. The impedance spectra of these four SAMs were fitted by a series of parallel combinations of a resistance and a capacitance (RC meshes) and displayed on plots of ωZ′ against −ωZ″, where Z′ and Z″ are the in-phase and quadrature components of the impedance and ω is the angular frequency. A comparison among these different impedance spectra points to the formation of GM1-rich gel phase microdomains within the lipid rafts of the DOPC/Chol/PSM mixture, thanks to the unique molecular-level smooth support provided by mercury, which allows EIS to detect the protruding gel phase microdomains by averaging them over a macroscopically large area.
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Becucci, L.; Vizza, F.; Duarte, Y.; Guidelli, R. The GM1 Ganglioside Forms GM1-Rich Gel Phase Microdomains within Lipid Rafts. Coatings 2014, 4, 450-464.
Becucci L, Vizza F, Duarte Y, Guidelli R. The GM1 Ganglioside Forms GM1-Rich Gel Phase Microdomains within Lipid Rafts. Coatings. 2014; 4(3):450-464.Chicago/Turabian Style
Becucci, Lucia; Vizza, Francesco; Duarte, Yolanda; Guidelli, Rolando. 2014. "The GM1 Ganglioside Forms GM1-Rich Gel Phase Microdomains within Lipid Rafts." Coatings 4, no. 3: 450-464.