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Coatings 2014, 4(3), 450-464; doi:10.3390/coatings4030450
Article

The GM1 Ganglioside Forms GM1-Rich Gel Phase Microdomains within Lipid Rafts

1,* , 1
, 2
 and 2,†
Received: 30 May 2014; in revised form: 1 July 2014 / Accepted: 7 July 2014 / Published: 16 July 2014
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Abstract: Mercury-supported, self-assembled monolayers (SAMs) of the sole dioleoylphosphatidylcholine (DOPC) and of a raft-forming mixture of DOPC, cholesterol (Chol) and palmitoylsphingomyelin (PSM) of (59:26:15) mol% composition, were investigated by electrochemical impedance spectroscopy (EIS), both in the absence and in the presence of the monosialoganglioside GM1. The impedance spectra of these four SAMs were fitted by a series of parallel combinations of a resistance and a capacitance (RC meshes) and displayed on plots of ωZ′ against −ωZ″, where Z′ and Z″ are the in-phase and quadrature components of the impedance and ω is the angular frequency. A comparison among these different impedance spectra points to the formation of GM1-rich gel phase microdomains within the lipid rafts of the DOPC/Chol/PSM mixture, thanks to the unique molecular-level smooth support provided by mercury, which allows EIS to detect the protruding gel phase microdomains by averaging them over a macroscopically large area.
Keywords: lipid rafts; gel phase microdomains; electrochemical impedance spectroscopy; cholera toxin lipid rafts; gel phase microdomains; electrochemical impedance spectroscopy; cholera toxin
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Becucci, L.; Vizza, F.; Duarte, Y.; Guidelli, R. The GM1 Ganglioside Forms GM1-Rich Gel Phase Microdomains within Lipid Rafts. Coatings 2014, 4, 450-464.

AMA Style

Becucci L, Vizza F, Duarte Y, Guidelli R. The GM1 Ganglioside Forms GM1-Rich Gel Phase Microdomains within Lipid Rafts. Coatings. 2014; 4(3):450-464.

Chicago/Turabian Style

Becucci, Lucia; Vizza, Francesco; Duarte, Yolanda; Guidelli, Rolando. 2014. "The GM1 Ganglioside Forms GM1-Rich Gel Phase Microdomains within Lipid Rafts." Coatings 4, no. 3: 450-464.


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