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Biosensors 2018, 8(3), 74; https://doi.org/10.3390/bios8030074

Cytokeratins Biosensing Using Tilted Fiber Gratings

1
Proteomics and Microbiology Department, University of Mons, Champ de Mars 6, 7000 Mons, Belgium
2
Department of Electronics, Carleton University, Mackenzie Building 5170, 1125 Colonel by Drive, Ottawa, ON K1S 5B6, Canada
3
Electromagnetism and Telecommunication Department, University of Mons, Bld Dolez 31, 7000 Mons, Belgium
*
Author to whom correspondence should be addressed.
Received: 5 July 2018 / Revised: 31 July 2018 / Accepted: 1 August 2018 / Published: 3 August 2018
(This article belongs to the Special Issue Immunoanalytical Tool with Electrochemical and Optical Detection)
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Abstract

Optical fiber gratings have widely proven their applicability in biosensing, especially when they are coupled with antibodies for specific antigen recognition. While this is customarily done with fibers coated by a thin metal film to benefit from plasmonic enhancement, in this paper, we propose to study their intrinsic properties, developing a label-free sensor for the detection of biomarkers in real-time without metal coatings for surface plasmon resonances. We focus on the inner properties of our modal sensor by immobilizing receptors directly on the silica surface, and reporting the sensitivity of bare tilted fiber Bragg gratings (TFBGs) used at near infrared wavelengths. We test different strategies to build our sensing surface against cytokeratins and show that the most reliable functionalization method is the electrostatic adsorption of antibodies on the fiber, allowing a limit of detection reaching 14 pM by following the guided cladding modes near the cut-off area. These results present the biodetection performance that TFBGs bring through their modal properties for different functionalizations and data processing strategies. View Full-Text
Keywords: optical fiber; fiber Bragg grating; immunosensing; cytokeratin; cancer biomarker; diagnosis optical fiber; fiber Bragg grating; immunosensing; cytokeratin; cancer biomarker; diagnosis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Loyez, M.; Albert, J.; Caucheteur, C.; Wattiez, R. Cytokeratins Biosensing Using Tilted Fiber Gratings. Biosensors 2018, 8, 74.

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