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Biosensors 2018, 8(3), 71; https://doi.org/10.3390/bios8030071

Label-Free Monitoring of Human IgG/Anti-IgG Recognition Using Bloch Surface Waves on 1D Photonic Crystals

1
Department of Basic and Applied Science for Engineering SBAI, Sapienza University of Rome, 00161 Rome, Italy
2
Fraunhofer Institute for Applied Optics and Precision Engineering IOF, Albert-Einstein-Str. 7, 07745 Jena, Germany
3
Fraunhofer Institute for Material and Beam Technology IWS, Winterbergstr. 28, 01277 Dresden, Germany
*
Author to whom correspondence should be addressed.
Received: 15 June 2018 / Revised: 18 July 2018 / Accepted: 23 July 2018 / Published: 25 July 2018
(This article belongs to the Special Issue Immunoanalytical Tool with Electrochemical and Optical Detection)
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Abstract

Optical biosensors based on one-dimensional photonic crystals sustaining Bloch surface waves are proposed to study antibody interactions and perform affinity studies. The presented approach utilizes two types of different antibodies anchored at the sensitive area of a photonic crystal-based biosensor. Such a strategy allows for creating two or more on-chip regions with different biochemical features as well as studying the binding kinetics of biomolecules in real time. In particular, the proposed detection system shows an estimated limit of detection for the target antibody (anti-human IgG) smaller than 0.19 nM (28 ng/mL), corresponding to a minimum surface mass coverage of 10.3 ng/cm2. Moreover, from the binding curves we successfully derived the equilibrium association and dissociation constants (KA = 7.5 × 107 M−1; KD = 13.26 nM) of the human IgG–anti-human IgG interaction. View Full-Text
Keywords: optical biosensors; one-dimensional photonic crystals; Bloch surface waves; human IgG screening optical biosensors; one-dimensional photonic crystals; Bloch surface waves; human IgG screening
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Sinibaldi, A.; Occhicone, A.; Munzert, P.; Danz, N.; Sonntag, F.; Michelotti, F. Label-Free Monitoring of Human IgG/Anti-IgG Recognition Using Bloch Surface Waves on 1D Photonic Crystals. Biosensors 2018, 8, 71.

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