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Biosensors 2018, 8(2), 37; https://doi.org/10.3390/bios8020037

Functionalized Thick Film Impedance Sensors for Use in In Vitro Cell Culture

1
Electronics Technology Group, Technische Universität Ilmenau, IMN MacroNano®, 98693 Ilmenau, Germany
2
Nano-Biosystems Technology Group, Technische Universität Ilmenau, IMN MacroNano®, 98693 Ilmenau, Germany
3
Institut de Neuroscience des Systèmes (INS), Aix-Marseille Université, 27 Bd Jean Moulin, CEDEX 13385 Marseille, France
4
Biomechatronics Group, Technische Universität Ilmenau, IMN MacroNano®, 98693 Ilmenau, Germany
*
Author to whom correspondence should be addressed.
Received: 13 March 2018 / Revised: 30 March 2018 / Accepted: 30 March 2018 / Published: 5 April 2018
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Abstract

Multi-electrode arrays find application in electrophysiological recordings. The quality of the captured signals depends on the interfacial contact between electrogenic cells and the electronic system. Therefore, it requires reliable low-impedance electrodes. Low-temperature cofired ceramic technology offers a suitable platform for rapid prototyping of biological reactors and can provide both stable fluid supply and integrated bio-hardware interfaces for recordings in electrogenic cell cultures. The 3D assembly of thick film gold electrodes in in vitro bio-reactors has been demonstrated for neuronal recordings. However, especially when dimensions become small, their performance varies strongly. This work investigates the influence of different coatings on thick film gold electrodes with regard to their influence on impedance behavior. PEDOT:PSS layer, titanium oxynitride and laminin coatings are deposited on LTCC gold electrodes using different 2D and 3D MEA chip designs. Their impedance characteristics are compared and discussed. Titanium oxynitride layers emerged as suitable functionalization. Small 86-µm-electrodes have a serial resistance Rs of 32 kOhm and serial capacitance Cs of 4.1 pF at 1 kHz. Thick film gold electrodes with such coatings are thus qualified for signal recording in 3-dimensional in vitro cell cultures. View Full-Text
Keywords: thick film sensors; impedance measurement; low temperature co-fired ceramics; functionalization; electrophysiological recordings thick film sensors; impedance measurement; low temperature co-fired ceramics; functionalization; electrophysiological recordings
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Bartsch, H.; Baca, M.; Fernekorn, U.; Müller, J.; Schober, A.; Witte, H. Functionalized Thick Film Impedance Sensors for Use in In Vitro Cell Culture. Biosensors 2018, 8, 37.

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