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Biosensors 2017, 7(4), 63; https://doi.org/10.3390/bios7040063

Fluorescence-Free Biosensor Methods in Detection of Food Pathogens with a Special Focus on Listeria monocytogenes

1
Faraday Technology Inc., Englewood, OH 45315, USA
2
ISPA-CNR, National Research Council, 73100 Lecce, Italy
*
Author to whom correspondence should be addressed.
Received: 13 October 2017 / Revised: 11 December 2017 / Accepted: 18 December 2017 / Published: 20 December 2017
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Abstract

Food pathogens contaminate food products that allow their growth on the shelf and also under refrigerated conditions. Therefore, it is of utmost importance to lower the limit of detection (LOD) of the method used and to obtain the results within hours to few days. Biosensor methods exploit the available technologies to individuate and provide an approximate quantification of the bacteria present in a sample. The main bottleneck of these methods depends on the aspecific binding to the surfaces and on a change in sensitivity when bacteria are in a complex food matrix with respect to bacteria in a liquid food sample. In this review, we introduce surface plasmon resonance (SPR), new advancements in SPR techniques, and electrochemical impedance spectroscopy (EIS), as fluorescence-free biosensing technologies for detection of L. monocytogenes in foods. The application of the two methods has facilitated L. monocytogenes detection with LOD of 1 log CFU/mL. Further advancements are envisaged through the combination of biosensor methods with immunoseparation of bacteria from larger volumes, application of lab-on-chip technologies, and EIS sensing methods for multiplex pathogen detection. Validation efforts are being conducted to demonstrate the robustness of detection, reproducibility and variability in multi-site installations. View Full-Text
Keywords: Listeria monocytogenes; fluorescencel-free biosensors; surface plasmon resonance (SPR); rlectrochemical impedance spectroscopy (EIS) Listeria monocytogenes; fluorescencel-free biosensors; surface plasmon resonance (SPR); rlectrochemical impedance spectroscopy (EIS)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Radhakrishnan, R.; Poltronieri, P. Fluorescence-Free Biosensor Methods in Detection of Food Pathogens with a Special Focus on Listeria monocytogenes. Biosensors 2017, 7, 63.

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