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Biosensors 2018, 8(1), 7; https://doi.org/10.3390/bios8010007

A Low-Cost Inkjet-Printed Aptamer-Based Electrochemical Biosensor for the Selective Detection of Lysozyme

1
Department of Electrical and Computer Engineering, University of New Hampshire, Durham, NH 03824, USA
2
Department of Chemical Engineering, University of New Hampshire, Durham, NH 03824, USA
3
Center for Advanced Materials and Manufacturing Innovation, University of New Hampshire, Durham, NH 03824, USA
*
Author to whom correspondence should be addressed.
Received: 30 November 2017 / Revised: 23 December 2017 / Accepted: 10 January 2018 / Published: 15 January 2018
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Abstract

Recently, inkjet-printing has gained increased popularity in applications such as flexible electronics and disposable sensors, as well as in wearable sensors because of its multifarious advantages. This work presents a novel, low-cost immobilization technique using inkjet-printing for the development of an aptamer-based biosensor for the detection of lysozyme, an important biomarker in various disease diagnosis. The strong affinity between the carbon nanotube (CNT) and the single-stranded DNA is exploited to immobilize the aptamers onto the working electrode by printing the ink containing the dispersion of CNT-aptamer complex. The inkjet-printing method enables aptamer density control, as well as high resolution patternability. Our developed sensor shows a detection limit of 90 ng/mL with high target selectivity against other proteins. The sensor also demonstrates a shelf-life for a reasonable period. This technology has potential for applications in developing low-cost point-of-care diagnostic testing kits for home healthcare. View Full-Text
Keywords: biosensor; aptamer; lysozyme; electrochemical impedance spectroscopy; inkjet printing; point-of-care biosensor; aptamer; lysozyme; electrochemical impedance spectroscopy; inkjet printing; point-of-care
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Khan, N.I.; Maddaus, A.G.; Song, E. A Low-Cost Inkjet-Printed Aptamer-Based Electrochemical Biosensor for the Selective Detection of Lysozyme. Biosensors 2018, 8, 7.

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