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Biosensors 2017, 7(4), 52; doi:10.3390/bios7040052

Ramanomics: New Omics Disciplines Using Micro Raman Spectrometry with Biomolecular Component Analysis for Molecular Profiling of Biological Structures

1
Institute for Lasers, Photonics and Biophotonics, University at Buffalo, State University of New York, Buffalo, NY 14260-3000, USA
2
Advanced Cytometry Instrumentation Systems, LLC, 640 Ellicott Street—Suite 499, Buffalo, NY 14203, USA
*
Authors to whom correspondence should be addressed.
Received: 17 September 2017 / Revised: 10 November 2017 / Accepted: 13 November 2017 / Published: 15 November 2017
(This article belongs to the Collection Raman and IR Spectroscopic Sensing)
View Full-Text   |   Download PDF [3432 KB, uploaded 15 November 2017]   |  

Abstract

Modern instrumentation for Raman microspectroscopy and current techniques in analysis of spectral data provide new opportunities to study molecular interactions and dynamics at subcellular levels in biological systems. Implementation of biomolecular component analysis (BCA) to microRaman spectrometry provides basis for the emergence of Ramanomics, a new biosensing discipline with unprecedented capabilities to measure concentrations of distinct biomolecular groups in live cells and organelles. Here we review the combined use of microRaman-BCA techniques to probe absolute concentrations of proteins, DNA, RNA and lipids in single organelles of live cells. Assessing biomolecular concentration profiles of organelles at the single cell level provides a physiologically relevant set of biomarkers for cellular heterogeneity. In addition, changes to an organelle’s biomolecular concentration profile during a cellular transformation, whether natural, drug induced or disease manifested, can provide molecular insight into the nature of the cellular process. View Full-Text
Keywords: Raman spectrometry; microscopy; biomolecular analysis; single cell assay Raman spectrometry; microscopy; biomolecular analysis; single cell assay
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Kuzmin, A.N.; Pliss, A.; Prasad, P.N. Ramanomics: New Omics Disciplines Using Micro Raman Spectrometry with Biomolecular Component Analysis for Molecular Profiling of Biological Structures. Biosensors 2017, 7, 52.

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