Next Article in Journal
Central [CNS] and Peripheral [Gastric Tissue] Selective Monitoring of Somatostatin (SRIF) with Micro-Sensor and Voltammetry in Rats: Influence of Growth Factors (GH, EGF)
Next Article in Special Issue
Study of SH-SY5Y Cancer Cell Response to Treatment with Polyphenol Extracts Using FT-IR Spectroscopy
Previous Article in Journal
Review on SERS of Bacteria
Previous Article in Special Issue
Raman Computational and Experimental Studies of Dopamine Detection
Article Menu
Issue 4 (December) cover image

Export Article

Open AccessReview
Biosensors 2017, 7(4), 52; doi:10.3390/bios7040052

Ramanomics: New Omics Disciplines Using Micro Raman Spectrometry with Biomolecular Component Analysis for Molecular Profiling of Biological Structures

Institute for Lasers, Photonics and Biophotonics, University at Buffalo, State University of New York, Buffalo, NY 14260-3000, USA
Advanced Cytometry Instrumentation Systems, LLC, 640 Ellicott Street—Suite 499, Buffalo, NY 14203, USA
Authors to whom correspondence should be addressed.
Received: 17 September 2017 / Revised: 10 November 2017 / Accepted: 13 November 2017 / Published: 15 November 2017
(This article belongs to the Collection Raman and IR Spectroscopic Sensing)
View Full-Text   |   Download PDF [3432 KB, uploaded 15 November 2017]   |  


Modern instrumentation for Raman microspectroscopy and current techniques in analysis of spectral data provide new opportunities to study molecular interactions and dynamics at subcellular levels in biological systems. Implementation of biomolecular component analysis (BCA) to microRaman spectrometry provides basis for the emergence of Ramanomics, a new biosensing discipline with unprecedented capabilities to measure concentrations of distinct biomolecular groups in live cells and organelles. Here we review the combined use of microRaman-BCA techniques to probe absolute concentrations of proteins, DNA, RNA and lipids in single organelles of live cells. Assessing biomolecular concentration profiles of organelles at the single cell level provides a physiologically relevant set of biomarkers for cellular heterogeneity. In addition, changes to an organelle’s biomolecular concentration profile during a cellular transformation, whether natural, drug induced or disease manifested, can provide molecular insight into the nature of the cellular process. View Full-Text
Keywords: Raman spectrometry; microscopy; biomolecular analysis; single cell assay Raman spectrometry; microscopy; biomolecular analysis; single cell assay

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Kuzmin, A.N.; Pliss, A.; Prasad, P.N. Ramanomics: New Omics Disciplines Using Micro Raman Spectrometry with Biomolecular Component Analysis for Molecular Profiling of Biological Structures. Biosensors 2017, 7, 52.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Biosensors EISSN 2079-6374 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top