Development of an Immunosensor for PfHRP 2 as a Biomarker for Malaria Detection
AbstractPlasmodium falciparum histidine-rich protein 2 (PfHRP 2) was selected in this work as the biomarker for the detection and diagnosis of malaria. An enzyme-linked immunosorbent assay (ELISA) was first developed to evaluate the immunoreagent’s suitability for the sensor’s development. A gold-based sensor with an integrated counter and an Ag/AgCl reference electrode was first selected and characterised and then used to develop the immunosensor for PfHRP 2, which enables a low cost, easy to use, and sensitive biosensor for malaria diagnosis. The sensor was applied to immobilise the anti-PfHRP 2 monoclonal antibody as the capture receptor. A sandwich ELISA assay format was constructed using horseradish peroxidase (HRP) as the enzyme label, and the electrochemical signal was generated using a 3, 3′, 5, 5′tetramethyl-benzidine dihydrochloride (TMB)/H2O2 system. The performance of the assay and the sensor were optimised and characterised, achieving a PfHRP 2 limit of detection (LOD) of 2.14 ng·mL−1 in buffer samples and 2.95 ng∙mL−1 in 100% spiked serum samples. The assay signal was then amplified using gold nanoparticles conjugated detection antibody-enzyme and a detection limit of 36 pg∙mL−1 was achieved in buffer samples and 40 pg∙mL−1 in serum samples. This sensor format is ideal for malaria detection and on-site analysis as a point-of-care device (POC) in resource-limited settings where the implementation of malaria diagnostics is essential in control and elimination efforts. View Full-Text
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Hemben, A.; Ashley, J.; Tothill, I.E. Development of an Immunosensor for PfHRP 2 as a Biomarker for Malaria Detection. Biosensors 2017, 7, 28.
Hemben A, Ashley J, Tothill IE. Development of an Immunosensor for PfHRP 2 as a Biomarker for Malaria Detection. Biosensors. 2017; 7(3):28.Chicago/Turabian Style
Hemben, Aver; Ashley, Jon; Tothill, Ibtisam E. 2017. "Development of an Immunosensor for PfHRP 2 as a Biomarker for Malaria Detection." Biosensors 7, no. 3: 28.
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