Next Article in Journal
A Label-Free and Ultrasensitive Immunosensor for Detection of Human Chorionic Gonadotrophin Based on Graphene FETs
Next Article in Special Issue
An Affordable Microsphere-Based Device for Visual Assessment of Water Quality
Previous Article in Journal
Detection of Lipid and Amphiphilic Biomarkers for Disease Diagnostics
Article Menu
Issue 3 (September) cover image

Export Article

Open AccessArticle
Biosensors 2017, 7(3), 26; doi:10.3390/bios7030026

Lipid Bilayer Membrane in a Silicon Based Micron Sized Cavity Accessed by Atomic Force Microscopy and Electrochemical Impedance Spectroscopy

1
Electrical and Computer Engineering Department, University of Alabama in Huntsville, Huntsville, AL 35899, USA
2
Biotechnology Science and Engineering Program, University of Alabama in Huntsville, Huntsville, AL 35899, USA
3
Department of Mathematics and Computer Science, Oakwood University, Huntsville, AL 35896, USA
4
Chemistry/Chemical and Materials Engineering Department, University of Alabama in Huntsville, Huntsville, AL 35899, USA
*
Author to whom correspondence should be addressed.
Received: 15 May 2017 / Revised: 27 June 2017 / Accepted: 30 June 2017 / Published: 5 July 2017
(This article belongs to the Special Issue Nanomaterials Based Optical Biosensors)
View Full-Text   |   Download PDF [2180 KB, uploaded 5 July 2017]   |  

Abstract

Supported lipid bilayers (SLBs) are widely used in biophysical research to probe the functionality of biological membranes and to provide diagnoses in high throughput drug screening. Formation of SLBs at below phase transition temperature (Tm) has applications in nano-medicine research where low temperature profiles are required. Herein, we report the successful production of SLBs at above—as well as below—the Tm of the lipids in an anisotropically etched, silicon-based micro-cavity. The Si-based cavity walls exhibit controlled temperature which assist in the quick and stable formation of lipid bilayer membranes. Fusion of large unilamellar vesicles was monitored in real time in an aqueous environment inside the Si cavity using atomic force microscopy (AFM), and the lateral organization of the lipid molecules was characterized until the formation of the SLBs. The stability of SLBs produced was also characterized by recording the electrical resistance and the capacitance using electrochemical impedance spectroscopy (EIS). Analysis was done in the frequency regime of 10−2–105 Hz at a signal voltage of 100 mV and giga-ohm sealed impedance was obtained continuously over four days. Finally, the cantilever tip in AFM was utilized to estimate the bilayer thickness and to calculate the rupture force at the interface of the tip and the SLB. We anticipate that a silicon-based, micron-sized cavity has the potential to produce highly-stable SLBs below their Tm. The membranes inside the Si cavity could last for several days and allow robust characterization using AFM or EIS. This could be an excellent platform for nanomedicine experiments that require low operating temperatures. View Full-Text
Keywords: lipid bilayer membrane; large unilamellar vesicles; silicon cavity; atomic force microscopy; electrochemical impedance spectroscopy. lipid bilayer membrane; large unilamellar vesicles; silicon cavity; atomic force microscopy; electrochemical impedance spectroscopy.
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Khan, M.S.; Dosoky, N.S.; Patel, D.; Weimer, J.; Williams, J.D. Lipid Bilayer Membrane in a Silicon Based Micron Sized Cavity Accessed by Atomic Force Microscopy and Electrochemical Impedance Spectroscopy. Biosensors 2017, 7, 26.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Biosensors EISSN 2079-6374 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top