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Biosensors 2016, 6(2), 24; doi:10.3390/bios6020024

Rapid Detection of Food Allergens by Microfluidics ELISA-Based Optical Sensor

Bionano Lab, School of Engineering, University of Guelph, Guelph, ON N1G 2W1, Canada
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Author to whom correspondence should be addressed.
Academic Editor: Samuel B. Adeloju
Received: 12 April 2016 / Revised: 19 May 2016 / Accepted: 2 June 2016 / Published: 7 June 2016
(This article belongs to the Special Issue Novel Nanobiosensors for Environmental, Food and Clinical Analyses)
View Full-Text   |   Download PDF [1505 KB, uploaded 7 June 2016]   |  

Abstract

The risks associated with the presence of hidden allergens in food have increased the need for rapid, sensitive, and reliable methods for tracing food allergens in commodities. Conventional enzyme immunosorbent assay (ELISA) has usually been performed in a centralized lab, requiring considerable time and sample/reagent consumption and expensive detection instruments. In this study, a microfluidic ELISA platform combined with a custom-designed optical sensor was developed for the quantitative analysis of the proteins wheat gluten and Ara h 1. The developed microfluidic ELISA biosensor reduced the total assay time from hours (up to 3.5 h) to 15–20 min and decreased sample/reagent consumption to 5–10 μL, compared to a few hundred microliters in commercial ELISA kits, with superior sensitivity. The quantitative capability of the presented biosensor is a distinctive advantage over the commercially available rapid methods such as lateral flow devices (LFD) and dipstick tests. The developed microfluidic biosensor demonstrates the potential for sensitive and less-expensive on-site determination for rapidly detecting food allergens in a complex sample system. View Full-Text
Keywords: food allergen; gluten; Ara h 1; microfluidics; optical sensor food allergen; gluten; Ara h 1; microfluidics; optical sensor
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Weng, X.; Gaur, G.; Neethirajan, S. Rapid Detection of Food Allergens by Microfluidics ELISA-Based Optical Sensor. Biosensors 2016, 6, 24.

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