Next Article in Journal
Rapid Detection of Food Allergens by Microfluidics ELISA-Based Optical Sensor
Next Article in Special Issue
Potential of Surface Enhanced Raman Spectroscopy (SERS) in Therapeutic Drug Monitoring (TDM). A Critical Review
Previous Article in Journal
A Flow SPR Immunosensor Based on a Sandwich Direct Method
Previous Article in Special Issue
Infrared Spectroscopy of Bilberry Extract Water-in-Oil Emulsions: Sensing the Water-Oil Interface
Article Menu

Export Article

Open AccessArticle
Biosensors 2016, 6(2), 23; doi:10.3390/bios6020023

Broadband 120 MHz Impedance Quartz Crystal Microbalance (QCM) with Calibrated Resistance and Quantitative Dissipation for Biosensing Measurements at Higher Harmonic Frequencies

1
Keysight Technologies Austria GmbH, Keysight Labs Linz, Gruberstrasse 40, 4020 Linz, Austria
2
Johannes Kepler University Linz, Biophysics Institute, Gruberstrasse 40, 4020 Linz, Austria
3
Faculty of Science, Department of Chemistry, Division of Physical Chemistry University of Zagreb, Horvatovac 102a, 10000 Zagreb, Croatia
4
University of Applied Sciences Upper Austria, School of Engineering, Stelzhamerstrasse 23, 4600 Wels, Austria
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Torsten Frosch
Received: 29 March 2016 / Revised: 13 May 2016 / Accepted: 16 May 2016 / Published: 25 May 2016
(This article belongs to the Special Issue Raman and IR Spectroscopic Sensing)
View Full-Text   |   Download PDF [3174 KB, uploaded 25 May 2016]   |  

Abstract

We developed an impedance quartz crystal microbalance (QCM) approach with the ability to simultaneously record mass changes and calibrated energy dissipation with high sensitivity using an impedance analyzer. This impedance QCM measures frequency shifts and resistance changes of sensing quartz crystals very stable, accurately, and calibrated, thus yielding quantitative information on mass changes and dissipation. Resistance changes below 0.3 Ω were measured with corresponding dissipation values of 0.01 µU (micro dissipation units). The broadband impedance capabilities allow measurements between 20 Hz and 120 MHz including higher harmonic modes of up to 11th order for a 10 MHz fundamental resonance frequency quartz crystal. We demonstrate the adsorbed mass, calibrated resistance, and quantitative dissipation measurements on two biological systems including the high affinity based avidin-biotin interaction and nano-assemblies of polyelectrolyte layers. The binding affinity of a protein-antibody interaction was determined. The impedance QCM is a versatile and simple method for accurate and calibrated resistance and dissipation measurements with broadband measurement capabilities for higher harmonics measurements. View Full-Text
Keywords: quartz crystal microbalance; impedance analysis; biosensing; dissipation; higher harmonics quartz crystal microbalance; impedance analysis; biosensing; dissipation; higher harmonics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Kasper, M.; Traxler, L.; Salopek, J.; Grabmayr, H.; Ebner, A.; Kienberger, F. Broadband 120 MHz Impedance Quartz Crystal Microbalance (QCM) with Calibrated Resistance and Quantitative Dissipation for Biosensing Measurements at Higher Harmonic Frequencies. Biosensors 2016, 6, 23.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Biosensors EISSN 2079-6374 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top