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Biosensors 2015, 5(4), 736-749; doi:10.3390/bios5040736

Microfluidic Impedimetric Cell Regeneration Assay to Monitor the Enhanced Cytotoxic Effect of Nanomaterial Perfusion

1
BioSensor Technologies, AIT Austrian Institute of Technology GmbH, 1190 Vienna, Austria
2
Molecular and Cellular Oncology, ENT/University Medical Center Mainz, 55116 Mainz, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Christophe A. Marquette
Received: 10 September 2015 / Revised: 28 October 2015 / Accepted: 24 November 2015 / Published: 27 November 2015
(This article belongs to the Special Issue Cell and Organ on Chip: Challenges and Advances)
View Full-Text   |   Download PDF [1028 KB, uploaded 27 November 2015]   |  

Abstract

In the last decade, the application of nanomaterials (NMs) in technical products and biomedicine has become a rapidly increasing market trend. As the safety and efficacy of NMs are of utmost importance, new methods are needed to study the dynamic interactions of NMs at the nano-biointerface. However, evaluation of NMs based on standard and static cell culture end-point detection methods does not provide information on the dynamics of living biological systems, which is crucial for the understanding of physiological responses. To bridge this technological gap, we here present a microfluidic cell culture system containing embedded impedance microsensors to continuously and non-invasively monitor the effects of NMs on adherent cells under varying flow conditions. As a model, the impact of silica NMs on the vitality and regenerative capacity of human lung cells after acute and chronic exposure scenarios was studied over an 18-h period following a four-hour NM treatment. Results of the study demonstrated that the developed system is applicable to reliably analyze the consequences of dynamic NM exposure to physiological cell barriers in both nanotoxicology and nanomedicine. View Full-Text
Keywords: lab-on-a-chip; cell chip; impedance; biosensor; nanoparticle; lung cancer; nanotoxicology lab-on-a-chip; cell chip; impedance; biosensor; nanoparticle; lung cancer; nanotoxicology
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Rothbauer, M.; Praisler, I.; Docter, D.; Stauber, R.H.; Ertl, P. Microfluidic Impedimetric Cell Regeneration Assay to Monitor the Enhanced Cytotoxic Effect of Nanomaterial Perfusion. Biosensors 2015, 5, 736-749.

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