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Biosensors 2015, 5(4), 750-767; doi:10.3390/bios5040750

A Simple Microfluidic Platform for Long-Term Analysis and Continuous Dual-Imaging Detection of T-Cell Secreted IFN-γ and IL-2 on Antibody-Based Biochip

1
University of Grenoble Alpes, INAC-SPRAM, Grenoble F-38000, France
2
CEA, INAC-SPrAM, Grenoble F-38000, France
3
CNRS, INAC-SPrAM, Grenoble F-38000, France
4
University Grenoble Alpes, Institut Albert Bonniot, Grenoble F-38000, France
5
INSERM U823, Grenoble F-38000, France
*
Author to whom correspondence should be addressed.
Academic Editor: Christophe A. Marquette
Received: 30 September 2015 / Revised: 12 November 2015 / Accepted: 25 November 2015 / Published: 4 December 2015
(This article belongs to the Special Issue Cell and Organ on Chip: Challenges and Advances)
View Full-Text   |   Download PDF [755 KB, uploaded 4 December 2015]   |  

Abstract

The identification and characterization, at the cellular level, of cytokine productions present a high interest for both fundamental research and clinical studies. However, the majority of techniques currently available (ELISA, ELISpot, flow cytometry, etc.) have several shortcomings including, notably, the assessment of several cytokines in relation to individual secreting cells and the monitoring of living cell responses for a long incubation time. In the present work, we describe a system composed of a microfluidic platform coupled with an antibody microarray chip for continuous SPR imaging and immunofluorescence analysis of cytokines (IL-2 and IFN-γ) secreted by T-Lymphocytes, specifically, and stably captured on the biochip under flow upon continued long-term on-chip culture (more than 24 h). View Full-Text
Keywords: Cytokine; T-Lymphocytes; biochip; microfluidics; antibody; PDMS; self-assembled monolayers; SPR imaging Cytokine; T-Lymphocytes; biochip; microfluidics; antibody; PDMS; self-assembled monolayers; SPR imaging
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Baganizi, D.R.; Leroy, L.; Laplatine, L.; Fairley, S.J.; Heidmann, S.; Menad, S.; Livache, T.; Marche, P.N.; Roupioz, Y. A Simple Microfluidic Platform for Long-Term Analysis and Continuous Dual-Imaging Detection of T-Cell Secreted IFN-γ and IL-2 on Antibody-Based Biochip. Biosensors 2015, 5, 750-767.

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