Next Article in Journal
Sensing of p53 and EGFR Biomarkers Using High Efficiency SERS Substrates
Next Article in Special Issue
Microfluidic Impedimetric Cell Regeneration Assay to Monitor the Enhanced Cytotoxic Effect of Nanomaterial Perfusion
Previous Article in Journal
In Vivo Electrochemical Analysis of a PEDOT/MWCNT Neural Electrode Coating
Previous Article in Special Issue
Design and Characterization of a Sensorized Microfluidic Cell-Culture System with Electro-Thermal Micro-Pumps and Sensors for Cell Adhesion, Oxygen, and pH on a Glass Chip
Article Menu

Export Article

Open AccessReview
Biosensors 2015, 5(4), 647-663; doi:10.3390/bios5040647

Biocompatible Hydrogels for Microarray Cell Printing and Encapsulation

Department of Chemical & Biomedical Engineering, Cleveland State University, 1960 East 24th Street Cleveland, OH 44115-2214, USA
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Christophe A. Marquette
Received: 28 September 2015 / Revised: 21 October 2015 / Accepted: 22 October 2015 / Published: 26 October 2015
(This article belongs to the Special Issue Cell and Organ on Chip: Challenges and Advances)
View Full-Text   |   Download PDF [327 KB, uploaded 26 October 2015]   |  

Abstract

Conventional drug screening processes are a time-consuming and expensive endeavor, but highly rewarding when they are successful. To identify promising lead compounds, millions of compounds are traditionally screened against therapeutic targets on human cells grown on the surface of 96-wells. These two-dimensional (2D) cell monolayers are physiologically irrelevant, thus, often providing false-positive or false-negative results, when compared to cells grown in three-dimensional (3D) structures such as hydrogel droplets. However, 3D cell culture systems are not easily amenable to high-throughput screening (HTS), thus inherently low throughput, and requiring relatively large volume for cell-based assays. In addition, it is difficult to control cellular microenvironments and hard to obtain reliable cell images due to focus position and transparency issues. To overcome these problems, miniaturized 3D cell cultures in hydrogels were developed via cell printing techniques where cell spots in hydrogels can be arrayed on the surface of glass slides or plastic chips by microarray spotters and cultured in growth media to form cells encapsulated 3D droplets for various cell-based assays. These approaches can dramatically reduce assay volume, provide accurate control over cellular microenvironments, and allow us to obtain clear 3D cell images for high-content imaging (HCI). In this review, several hydrogels that are compatible to microarray printing robots are discussed for miniaturized 3D cell cultures. View Full-Text
Keywords: microarray; cell encapsulation; hydrogel; bioprinting; miniaturized 3D cell culture microarray; cell encapsulation; hydrogel; bioprinting; miniaturized 3D cell culture
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Datar, A.; Joshi, P.; Lee, M.-Y. Biocompatible Hydrogels for Microarray Cell Printing and Encapsulation. Biosensors 2015, 5, 647-663.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Biosensors EISSN 2079-6374 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top