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Biosensors 2015, 5(4), 664-677; doi:10.3390/bios5040664

Sensing of p53 and EGFR Biomarkers Using High Efficiency SERS Substrates

Centre for Microscopy and Imaging, National University Ireland, University Road, Galway, Ireland
School of Physics, National University Ireland, University Road, Galway, Ireland
Bio-Optical Imaging Group, Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), 11 Biopolis Way, #02-02 Helios 138667, Singapore
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Academic Editor: Yuliya Semenova
Received: 3 September 2015 / Revised: 8 October 2015 / Accepted: 20 October 2015 / Published: 28 October 2015
(This article belongs to the Special Issue Optical Sensors for Biomedical Applications)
View Full-Text   |   Download PDF [1098 KB, uploaded 2 November 2015]   |  


In this paper we describe a method for the determination of protein concentration using Surface Enhanced Raman Resonance Scattering (SERRS) immunoassays. We use two different Raman active linkers, 4-aminothiophenol and 6-mercaptopurine, to bind to a high sensitivity SERS substrate and investigate the influence of varying concentrations of p53 and EGFR on the Raman spectra. Perturbations in the spectra are due to the influence of protein–antibody binding on Raman linker molecules and are attributed to small changes in localised mechanical stress, which are enhanced by SERRS. These influences are greatest for peaks due to the C-S functional group and the Full Width Half Maximum (FWHM) was found to be inversely proportional to protein concentration. View Full-Text
Keywords: Raman spectroscopy; protein sensing; surface-enhanced Raman scattering; biomarker Raman spectroscopy; protein sensing; surface-enhanced Raman scattering; biomarker

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Owens, P.; Phillipson, N.; Perumal, J.; O’Connor, G.M.; Olivo, M. Sensing of p53 and EGFR Biomarkers Using High Efficiency SERS Substrates. Biosensors 2015, 5, 664-677.

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