Next Article in Journal
Escherichia coli O-Antigen Gene Clusters of Serogroups O62, O68, O131, O140, O142, and O163: DNA Sequences and Similarity between O62 and O68, and PCR-Based Serogrouping
Next Article in Special Issue
Label-Free Sensing of Adenosine Based on Force Variations Induced by Molecular Recognition
Previous Article in Journal
Kinetic Analyses of Data from a Human Serum Albumin Assay Using the liSPR System
Previous Article in Special Issue
Tracking Traction Force Changes of Single Cells on the Liquid Crystal Surface
Article Menu

Export Article

Open AccessArticle
Biosensors 2015, 5(1), 37-50; doi:10.3390/bios5010037

Direct Determination of a Small-Molecule Drug, Valproic Acid, by an Electrically-Detected Microcantilever Biosensor for Personalized Diagnostics

1
Institute of Applied Mechanics, National Taiwan University, Taipei 10617, Taiwan
2
Department of Chemical Physics, Weizmann Institute of Science, 234 Herzl Street, Rehovot 7610001, Israel
*
Author to whom correspondence should be addressed.
Academic Editor: Andrew Shaw
Received: 14 November 2014 / Revised: 17 December 2014 / Accepted: 21 January 2015 / Published: 27 January 2015
(This article belongs to the Special Issue Label-Free Biosensors: Exploring the Field)
View Full-Text   |   Download PDF [639 KB, uploaded 27 January 2015]   |  

Abstract

Direct, small-molecule determination of the antiepileptic drug, valproic acid, was investigated by a label-free, nanomechanical biosensor. Valproic acid has long been used as an antiepileptic medication, which is administered through therapeutic drug monitoring and has a narrow therapeutic dosage range of 50–100 μg·mL1 in blood or serum. Unlike labeled and clinically-used measurement techniques, the label-free, electrical detection microcantilever biosensor can be miniaturized and simplified for use in portable or hand-held point-of-care platforms or personal diagnostic tools. A micromachined microcantilever sensor was packaged into the micro-channel of a fluidic system. The measurement of the antiepileptic drug, valproic acid, in phosphate-buffered saline and serum used a single free-standing, piezoresistive microcantilever biosensor in a thermally-controlled system. The measured surface stresses showed a profile over a concentration range of 50–500 μg·mL1, which covered the clinically therapeutic range of 50–100 μg·mL1. The estimated limit of detection (LOD) was calculated to be 45 μg·mL−1, and the binding affinity between the drug and the antibody was measured at around 90 ± 21 μg·mL1. Lastly, the results of the proposed device showed a similar profile in valproic acid drug detection with those of the clinically-used fluorescence polarization immunoassay. View Full-Text
Keywords: valproic acid; microcantilever; serum; therapeutic drug monitoring valproic acid; microcantilever; serum; therapeutic drug monitoring
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Huang, L.-S.; Gunawan, C.; Yen, Y.-K.; Chang, K.-F. Direct Determination of a Small-Molecule Drug, Valproic Acid, by an Electrically-Detected Microcantilever Biosensor for Personalized Diagnostics. Biosensors 2015, 5, 37-50.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Biosensors EISSN 2079-6374 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top