Next Article in Journal
Direct Determination of a Small-Molecule Drug, Valproic Acid, by an Electrically-Detected Microcantilever Biosensor for Personalized Diagnostics
Previous Article in Journal
Acknowledgement to Reviewers of Biosensors in 2014
Article Menu

Export Article

Open AccessArticle
Biosensors 2015, 5(1), 27-36; doi:10.3390/bios5010027

Kinetic Analyses of Data from a Human Serum Albumin Assay Using the liSPR System

1
Institute of Food Technology and Bioprocess Engineering, Technische Universität Dresden, Dresden 01062, Germany
2
QuoData GmbH, Prellerstraße 14, Dresden 01309, Germany
3
New diagnostics GmbH, Moosstraße 92c, Freising D-85356, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Jeff D. Newman
Received: 9 September 2014 / Revised: 26 September 2014 / Accepted: 19 December 2014 / Published: 19 January 2015
View Full-Text   |   Download PDF [633 KB, uploaded 19 January 2015]   |  

Abstract

We used the interaction between human serum albumin (HSA) and a high-affinity antibody to evaluate binding affinity measurements by the bench-top liSPR system (capitalis technology GmbH). HSA was immobilized directly onto a carboxylated sensor layer, and the mechanism of interaction between the antibody and HSA was investigated. The bivalence and heterogeneity of the antibody caused a complex binding mechanism. Three different interaction models (1:1 binding, heterogeneous analyte, bivalent analyte) were compared, and the bivalent analyte model best fit the curves obtained from the assay. This model describes the interaction of a bivalent analyte with one or two ligands (A + L ↔ LA + L ↔ LLA). The apparent binding affinity for this model measured 37 pM for the first reaction step, and 20 pM for the second step. View Full-Text
Keywords: surface plasmon resonance (SPR); human serum albumin (HSA); antibody; bivalent analyte surface plasmon resonance (SPR); human serum albumin (HSA); antibody; bivalent analyte
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Henseleit, A.; Pohl, C.; Kaltenbach, H.-M.; Hettwer, K.; Simon, K.; Uhlig, S.; Haustein, N.; Bley, T.; Boschke, E. Kinetic Analyses of Data from a Human Serum Albumin Assay Using the liSPR System. Biosensors 2015, 5, 27-36.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Biosensors EISSN 2079-6374 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top