Biosensors 2014, 4(1), 76-89; doi:10.3390/bios4010076
Article

Asynchronous Magnetic Bead Rotation (AMBR) Microviscometer for Label-Free DNA Analysis

1email, 2email, 3,4,* email and 1,4,* email
Received: 17 January 2014; in revised form: 27 February 2014 / Accepted: 17 March 2014 / Published: 21 March 2014
(This article belongs to the Special Issue Magnetic Biosensors)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: We have developed a label-free viscosity-based DNA detection system, using paramagnetic beads as an asynchronous magnetic bead rotation (AMBR) microviscometer. We have demonstrated experimentally that the bead rotation period is linearly proportional to the viscosity of a DNA solution surrounding the paramagnetic bead, as expected theoretically. Simple optical measurement of asynchronous microbead motion determines solution viscosity precisely in microscale volumes, thus allowing an estimate of DNA concentration or average fragment length. The response of the AMBR microviscometer yields reproducible measurement of DNA solutions, enzymatic digestion reactions, and PCR systems at template concentrations across a 5000-fold range. The results demonstrate the feasibility of viscosity-based DNA detection using AMBR in microscale aqueous volumes.
Keywords: label-free; DNA; quantitative PCR; restriction digestion; genetic analysis; viscosity; viscometer; magnetic; paramagnetic; microdevice
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MDPI and ACS Style

Li, Y.; Burke, D.T.; Kopelman, R.; Burns, M.A. Asynchronous Magnetic Bead Rotation (AMBR) Microviscometer for Label-Free DNA Analysis. Biosensors 2014, 4, 76-89.

AMA Style

Li Y, Burke DT, Kopelman R, Burns MA. Asynchronous Magnetic Bead Rotation (AMBR) Microviscometer for Label-Free DNA Analysis. Biosensors. 2014; 4(1):76-89.

Chicago/Turabian Style

Li, Yunzi; Burke, David T.; Kopelman, Raoul; Burns, Mark A. 2014. "Asynchronous Magnetic Bead Rotation (AMBR) Microviscometer for Label-Free DNA Analysis." Biosensors 4, no. 1: 76-89.

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