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Single Step Nanoplasmonic Immunoassay for the Measurement of Protein Biomarkers
Department of Ophthalmology, University of Miami-Bascom Palmer Eye Institute, Miami, FL 33136, USA
Department of Structural Biology, Stanford University, Palo Alto, CA 94305, USA
* Author to whom correspondence should be addressed.
Received: 11 December 2012; in revised form: 18 January 2013 / Accepted: 1 February 2013 / Published: 6 February 2013
Abstract: A nanoplasmonic biosensor for highly-sensitive, single-step detection of protein biomarkers is presented. The principle is based on the utilization of the optical scattering properties of gold nanorods (GNRs) conjugated to bio-recognition molecules. The nanoplasmonic properties of the GNRs were utilized to detect proteins using near-infrared light interferometry. We show that the antibody-conjugated GNRs can specifically bind to our model analyte, Glucose Transporter-1 (Glut-1). The signal intensity of back-scattered light from the GNRs bound after incubation, correlated well to the Glut-1 concentration as per the calibration curve. The detection range using this nanoplasmonic immunoassay ranges from 10 ng/mL to 1 ug/mL for Glut-1. The minimal detectable concentration based on the lowest discernable concentration from zero is 10 ng/mL. This nanoplasmonic immunoassay can act as a simple, selective, sensitive strategy for effective disease diagnosis. It offers advantages such as wide detection range, increased speed of analysis (due to fewer incubation/washing steps), and no label development as compared to traditional immunoassay techniques. Our future goal is to incorporate this detection strategy onto a microfluidic platform to be used as a point-of-care diagnostic tool.
Keywords: gold nanorods; optical coherence tomography; protein biomarkers; immunoassay; surface plasmon resonance; glucose transporter-17
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Prabhulkar, S.; de la Zerda, A.; Paranjape, A.; Awdeh, R.M. Single Step Nanoplasmonic Immunoassay for the Measurement of Protein Biomarkers. Biosensors 2013, 3, 77-88.
Prabhulkar S, de la Zerda A, Paranjape A, Awdeh RM. Single Step Nanoplasmonic Immunoassay for the Measurement of Protein Biomarkers. Biosensors. 2013; 3(1):77-88.
Prabhulkar, Shradha; de la Zerda, Adam; Paranjape, Amit; Awdeh, Richard M. 2013. "Single Step Nanoplasmonic Immunoassay for the Measurement of Protein Biomarkers." Biosensors 3, no. 1: 77-88.