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• Ruckstuhl, T
• Seeger, S
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• Seeger, S
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• Ruckstuhl, T
• Seeger, S

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Biosensors 2013, 3(1), 108-115; doi:10.3390/bios3010108

Article

Fast and Sensitive Interferon-γ Assay Using Supercritical Angle Fluorescence

Christian M. Winterflood, Thomas Ruckstuhl and Stefan Seeger*

Physikalisch-Chemisches Institut, Universität Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland

* Author to whom correspondence should be addressed.

Received: 7 January 2013; in revised form: 28 January 2013 / Accepted: 7 February 2013 / Published: 8 February 2013

(This article belongs to the Special Issue Immunosensors 2012)

Download PDF Full-Text [377 KB, uploaded 8 February 2013 11:24 CET]

Abstract: We present an immunoassay for Interferon-γ (IFN-γ) with a limit of detection of 1.9 pM (30 pg/mL) and a linear concentration range spanning three orders of magnitude. The developed one-step assay takes only 12 min and can replace the time-consuming and labor-intensive enzyme-linked immunosorbent assay (ELISA). The solid-phase sandwich assay is performed on a new measurement system comprising single-use test tubes and a compact fluorescence reader. The polymer tubes contain an optical configuration for the detection of supercritical angle fluorescence, allowing for highly sensitive real-time binding measurements.

Keywords: one-step immunoassay; interferon-gamma; supercritical angle fluorescence; polymer test tube

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