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Biosensors 2013, 3(1), 108-115; doi:10.3390/bios3010108
Article

Fast and Sensitive Interferon-γ Assay Using Supercritical Angle Fluorescence

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Received: 7 January 2013; in revised form: 28 January 2013 / Accepted: 7 February 2013 / Published: 8 February 2013
(This article belongs to the Special Issue Immunosensors 2012)
View Full-Text   |   Download PDF [377 KB, uploaded 8 February 2013]
Abstract: We present an immunoassay for Interferon-γ (IFN-γ) with a limit of detection of 1.9 pM (30 pg/mL) and a linear concentration range spanning three orders of magnitude. The developed one-step assay takes only 12 min and can replace the time-consuming and labor-intensive enzyme-linked immunosorbent assay (ELISA). The solid-phase sandwich assay is performed on a new measurement system comprising single-use test tubes and a compact fluorescence reader. The polymer tubes contain an optical configuration for the detection of supercritical angle fluorescence, allowing for highly sensitive real-time binding measurements.
Keywords: one-step immunoassay; interferon-gamma; supercritical angle fluorescence; polymer test tube one-step immunoassay; interferon-gamma; supercritical angle fluorescence; polymer test tube
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Winterflood, C.M.; Ruckstuhl, T.; Seeger, S. Fast and Sensitive Interferon-γ Assay Using Supercritical Angle Fluorescence. Biosensors 2013, 3, 108-115.

AMA Style

Winterflood CM, Ruckstuhl T, Seeger S. Fast and Sensitive Interferon-γ Assay Using Supercritical Angle Fluorescence. Biosensors. 2013; 3(1):108-115.

Chicago/Turabian Style

Winterflood, Christian M.; Ruckstuhl, Thomas; Seeger, Stefan. 2013. "Fast and Sensitive Interferon-γ Assay Using Supercritical Angle Fluorescence." Biosensors 3, no. 1: 108-115.

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