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Fast and Sensitive Interferon-γ Assay Using Supercritical Angle Fluorescence
Physikalisch-Chemisches Institut, Universität Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland
* Author to whom correspondence should be addressed.
Received: 7 January 2013; in revised form: 28 January 2013 / Accepted: 7 February 2013 / Published: 8 February 2013
Abstract: We present an immunoassay for Interferon-γ (IFN-γ) with a limit of detection of 1.9 pM (30 pg/mL) and a linear concentration range spanning three orders of magnitude. The developed one-step assay takes only 12 min and can replace the time-consuming and labor-intensive enzyme-linked immunosorbent assay (ELISA). The solid-phase sandwich assay is performed on a new measurement system comprising single-use test tubes and a compact fluorescence reader. The polymer tubes contain an optical configuration for the detection of supercritical angle fluorescence, allowing for highly sensitive real-time binding measurements.
Keywords: one-step immunoassay; interferon-gamma; supercritical angle fluorescence; polymer test tube
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Cite This Article
MDPI and ACS Style
Winterflood, C.M.; Ruckstuhl, T.; Seeger, S. Fast and Sensitive Interferon-γ Assay Using Supercritical Angle Fluorescence. Biosensors 2013, 3, 108-115.
Winterflood CM, Ruckstuhl T, Seeger S. Fast and Sensitive Interferon-γ Assay Using Supercritical Angle Fluorescence. Biosensors. 2013; 3(1):108-115.
Winterflood, Christian M.; Ruckstuhl, Thomas; Seeger, Stefan. 2013. "Fast and Sensitive Interferon-γ Assay Using Supercritical Angle Fluorescence." Biosensors 3, no. 1: 108-115.