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Biosensors 2013, 3(1), 1-17; doi:10.3390/bios3010001
Article

Application of Paper-Supported Printed Gold Electrodes for Impedimetric Immunosensor Development

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Received: 15 November 2012; in revised form: 13 December 2012 / Accepted: 24 December 2012 / Published: 27 December 2012
(This article belongs to the Special Issue Organic Electronic Bio-Devices)
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Abstract: In this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRP antibody are grown on printed gold electrodes on disposable paper-substrates. We have successfully demonstrated and detected the formation of consecutive layers of supra-molecular protein assembly using an electrical (impedimetric) technique. The formation process is also supplemented and verified using conventional surface plasmon resonance (SPR) measurements and surface sensitive characterization techniques, such as X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The article provides a possible biosensor development scheme, where—(1) fabrication of paper substrate (2) synthesis of gold nanoparticle inks (3) inkjet printing of gold electrodes on paper (4) formation of the biorecognition layers on the gold electrodes and (5) electrical (impedimetric) analysis of growth—all are coupled together to form a test-structure for a recyclable and inexpensive point-of-care diagnostic platform.
Keywords: paper electronics; nanoparticles; inkjet printing; immunoassays; impedance spectroscopy paper electronics; nanoparticles; inkjet printing; immunoassays; impedance spectroscopy
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Ihalainen, P.; Majumdar, H.; Viitala, T.; Törngren, B.; Närjeoja, T.; Määttänen, A.; Sarfraz, J.; Härmä, H.; Yliperttula, M.; Österbacka, R.; Peltonen, J. Application of Paper-Supported Printed Gold Electrodes for Impedimetric Immunosensor Development. Biosensors 2013, 3, 1-17.

AMA Style

Ihalainen P, Majumdar H, Viitala T, Törngren B, Närjeoja T, Määttänen A, Sarfraz J, Härmä H, Yliperttula M, Österbacka R, Peltonen J. Application of Paper-Supported Printed Gold Electrodes for Impedimetric Immunosensor Development. Biosensors. 2013; 3(1):1-17.

Chicago/Turabian Style

Ihalainen, Petri; Majumdar, Himadri; Viitala, Tapani; Törngren, Björn; Närjeoja, Tuomas; Määttänen, Anni; Sarfraz, Jawad; Härmä, Harri; Yliperttula, Marjo; Österbacka, Ronald; Peltonen, Jouko. 2013. "Application of Paper-Supported Printed Gold Electrodes for Impedimetric Immunosensor Development." Biosensors 3, no. 1: 1-17.


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