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Biosensors 2013, 3(1), 18-43; doi:10.3390/bios3010018
Review

Isothermal Amplification Methods for the Detection of Nucleic Acids in Microfluidic Devices

1
 and 1,2,*
Received: 7 November 2012; in revised form: 7 December 2012 / Accepted: 24 December 2012 / Published: 27 December 2012
(This article belongs to the Special Issue Organic Electronic Bio-Devices)
View Full-Text   |   Download PDF [1077 KB, uploaded 27 December 2012]
Abstract: Diagnostic tools for biomolecular detection need to fulfill specific requirements in terms of sensitivity, selectivity and high-throughput in order to widen their applicability and to minimize the cost of the assay. The nucleic acid amplification is a key step in DNA detection assays. It contributes to improving the assay sensitivity by enabling the detection of a limited number of target molecules. The use of microfluidic devices to miniaturize amplification protocols reduces the required sample volume and the analysis times and offers new possibilities for the process automation and integration in one single device. The vast majority of miniaturized systems for nucleic acid analysis exploit the polymerase chain reaction (PCR) amplification method, which requires repeated cycles of three or two temperature-dependent steps during the amplification of the nucleic acid target sequence. In contrast, low temperature isothermal amplification methods have no need for thermal cycling thus requiring simplified microfluidic device features. Here, the use of miniaturized analysis systems using isothermal amplification reactions for the nucleic acid amplification will be discussed.
Keywords: microfluidics; isothermal amplification methods; miniaturization; DNA microfluidics; isothermal amplification methods; miniaturization; DNA
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Zanoli, L.M.; Spoto, G. Isothermal Amplification Methods for the Detection of Nucleic Acids in Microfluidic Devices. Biosensors 2013, 3, 18-43.

AMA Style

Zanoli LM, Spoto G. Isothermal Amplification Methods for the Detection of Nucleic Acids in Microfluidic Devices. Biosensors. 2013; 3(1):18-43.

Chicago/Turabian Style

Zanoli, Laura M.; Spoto, Giuseppe. 2013. "Isothermal Amplification Methods for the Detection of Nucleic Acids in Microfluidic Devices." Biosensors 3, no. 1: 18-43.

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