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Synthesis of a Functionalized Polypyrrole Coated Electrotextile for Use in Biosensors
Biosystems and Agricultural Engineering, Michigan State University, 524 S. Shaw Lane, 115 Farrall Hall, East Lansing, MI 48824, USA
Food Protection Team, US Army Natick Soldier Research, Development, and Engineering Center (NSRDEC), Natick, MA 01760, USA
Macromolecular Sciences and Engineering Team, US Army NSRDEC, Natick, MA 01760, USA
* Author to whom correspondence should be addressed.
Received: 20 October 2012; in revised form: 15 November 2012 / Accepted: 20 November 2012 / Published: 29 November 2012
Abstract: An electrotextile with a biosensing focus composed of conductive polymer coated microfibers that contain functional attachment sites for biorecognition elements was developed. Experiments were conducted to select a compound with a pendant functional group for inclusion in the polymer, a fiber platform, and polymerization solvent. The effects of dopant inclusion and post-polymerization wash steps were also analyzed. Finally, the successful attachment of avidin, which was then used to capture biotin, to the electrotextile was achieved. The initial results show a nonwoven fiber matrix can be successfully coated in a conductive, functionalized polymer while still maintaining surface area and fiber durability. A polypropylene fiber platform with a conductive polypyrrole coating using iron (III) chloride as an oxidant, water as a solvent, and 5-sulfosalicylic acid as a dopant exhibited the best coating consistency, material durability, and lowest resistance. Biological attachment of avidin was achieved on the fibers through the inclusion of a carboxyl functional group via 3-thiopheneacetic acid in the monomer. The immobilized avidin was then successfully used to capture biotin. This was confirmed through the use of fluorescent quantum dots and confocal microscopy. A preliminary electrochemical experiment using avidin for biotin detection was conducted. This technology will be extremely useful in the formation of electrotextiles for use in biosensor systems.
Keywords: electrotextile; biosensor; polypyrrole; immobilization
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MDPI and ACS Style
McGraw, S.K.; Alocilja, E.; Senecal, A.; Senecal, K. Synthesis of a Functionalized Polypyrrole Coated Electrotextile for Use in Biosensors. Biosensors 2012, 2, 465-478.
McGraw SK, Alocilja E, Senecal A, Senecal K. Synthesis of a Functionalized Polypyrrole Coated Electrotextile for Use in Biosensors. Biosensors. 2012; 2(4):465-478.
McGraw, Shannon K.; Alocilja, Evangelyn; Senecal, Andre; Senecal, Kris. 2012. "Synthesis of a Functionalized Polypyrrole Coated Electrotextile for Use in Biosensors." Biosensors 2, no. 4: 465-478.