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Biosensors 2012, 2(4), 465-478; doi:10.3390/bios2040465
Article

Synthesis of a Functionalized Polypyrrole Coated Electrotextile for Use in Biosensors

1,2
,
1,* , 2
 and
3
1 Biosystems and Agricultural Engineering, Michigan State University, 524 S. Shaw Lane, 115 Farrall Hall, East Lansing, MI 48824, USA 2 Food Protection Team, US Army Natick Soldier Research, Development, and Engineering Center (NSRDEC), Natick, MA 01760, USA 3 Macromolecular Sciences and Engineering Team, US Army NSRDEC, Natick, MA 01760, USA
* Author to whom correspondence should be addressed.
Received: 20 October 2012 / Revised: 15 November 2012 / Accepted: 20 November 2012 / Published: 29 November 2012
(This article belongs to the Special Issue Organic Electronic Bio-Devices)
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Abstract

An electrotextile with a biosensing focus composed of conductive polymer coated microfibers that contain functional attachment sites for biorecognition elements was developed. Experiments were conducted to select a compound with a pendant functional group for inclusion in the polymer, a fiber platform, and polymerization solvent. The effects of dopant inclusion and post-polymerization wash steps were also analyzed. Finally, the successful attachment of avidin, which was then used to capture biotin, to the electrotextile was achieved. The initial results show a nonwoven fiber matrix can be successfully coated in a conductive, functionalized polymer while still maintaining surface area and fiber durability. A polypropylene fiber platform with a conductive polypyrrole coating using iron (III) chloride as an oxidant, water as a solvent, and 5-sulfosalicylic acid as a dopant exhibited the best coating consistency, material durability, and lowest resistance. Biological attachment of avidin was achieved on the fibers through the inclusion of a carboxyl functional group via 3-thiopheneacetic acid in the monomer. The immobilized avidin was then successfully used to capture biotin. This was confirmed through the use of fluorescent quantum dots and confocal microscopy. A preliminary electrochemical experiment using avidin for biotin detection was conducted. This technology will be extremely useful in the formation of electrotextiles for use in biosensor systems.
Keywords: electrotextile; biosensor; polypyrrole; immobilization electrotextile; biosensor; polypyrrole; immobilization
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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McGraw, S.K.; Alocilja, E.; Senecal, A.; Senecal, K. Synthesis of a Functionalized Polypyrrole Coated Electrotextile for Use in Biosensors. Biosensors 2012, 2, 465-478.

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