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Biosensors 2012, 2(4), 388-395; doi:10.3390/bios2040388

Water-Soluble Electrospun Nanofibers as a Method for On-Chip Reagent Storage

1 University of Massachusetts, 102 Holdsworth Way, Amherst, MA 01003, USA 2 Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai 200237, China
* Author to whom correspondence should be addressed.
Received: 15 August 2012 / Revised: 15 September 2012 / Accepted: 28 September 2012 / Published: 8 October 2012
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This work demonstrates the ability to electrospin reagents into water-soluble nanofibers resulting in a stable on-chip enzyme storage format. Polyvinylpyrrolidone (PVP) nanofibers were spun with incorporation of the enzyme horseradish peroxidase (HRP). Scanning electron microscopy (SEM) of the spun nanofibers was used to confirm the non-woven structure which had an average diameter of 155 ± 34 nm. The HRP containing fibers were tested for their change in activity following electrospinning and during storage. A colorimetric assay was used to characterize the activity of HRP by reaction with the nanofiber mats in a microtiter plate and monitoring the change in absorption over time. Immediately following electrospinning, the activity peak for the HRP decreased by approximately 20%. After a storage study over 280 days, 40% of the activity remained. In addition to activity, the fibers were observed to solubilize in the microfluidic chamber. The chromogenic 3,3′,5,5′-tetramethylbenzidine solution reacted immediately with the fibers as they passed through a microfluidic channel. The ability to store enzymes and other reagents on-chip in a rapidly dispersible format could reduce the assay steps required of an operator to perform.
Keywords: polyvinylpyrrolidone (PVP); nanofiber; electrospinning; microfluidic biosensor; enzyme storage polyvinylpyrrolidone (PVP); nanofiber; electrospinning; microfluidic biosensor; enzyme storage
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Dai, M.; Jin, S.; Nugen, S.R. Water-Soluble Electrospun Nanofibers as a Method for On-Chip Reagent Storage. Biosensors 2012, 2, 388-395.

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