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Nanomaterials 2016, 6(11), 199; doi:10.3390/nano6110199

Targeting at the Nanoscale: A Novel S-Layer Fusion Protein Enabling Controlled Immobilization of Biotinylated Molecules

Electronics Packaging Laboratory, Department of Electrical Engineering and Information Technology, Technische Universität Dresden, Dresden 01069, Germany
Academic Editors: Alina Holban and Alexandru Grumezescu
Received: 1 September 2016 / Revised: 25 October 2016 / Accepted: 28 October 2016 / Published: 4 November 2016
(This article belongs to the Special Issue Nanoarchitectonics: A Novel Approach for Drug Delivery and Targeting)
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Abstract

With the aim of constructing an S-layer fusion protein that combines both excellent self-assembly and specific ligand i.e., biotin binding ability, streptavidin (aa 16-133) was fused to the S-layer protein of Sporosarcina ureae ATCC 13881 (SslA) devoid of its N-terminal 341 and C-terminal 172 amino acids. The genetically engineered chimeric protein could be successfully produced in E. coli, isolated, and purified via Ni affinity chromatography. In vitro recrystallisation experiments performed with the purified chimeric protein in solution and on a silicon wafer have demonstrated that fusion of the streptavidin domain does not interfere with the self-assembling properties of the S-layer part. The chimeric protein self-assembled into multilayers. More importantly, the streptavidin domain retained its full biotin-binding ability, a fact evidenced by experiments in which biotinylated quantum dots were coupled to the fusion protein monomers and adsorbed onto the in vitro recrystallised fusion protein template. In this way, this S-layer fusion protein can serve as a functional template for the controlled immobilization of biotinylated and biologically active molecules. View Full-Text
Keywords: surface layer protein; Sporosarcina ureae ATCC 13881; quantum dots; self-assembly; biotinylated molecules surface layer protein; Sporosarcina ureae ATCC 13881; quantum dots; self-assembly; biotinylated molecules
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Varga, M. Targeting at the Nanoscale: A Novel S-Layer Fusion Protein Enabling Controlled Immobilization of Biotinylated Molecules. Nanomaterials 2016, 6, 199.

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