Next Article in Journal
Controllable Synthesis of Copper Oxide/Carbon Core/Shell Nanowire Arrays and Their Application for Electrochemical Energy Storage
Next Article in Special Issue
Gold Nanotheranostics: Proof-of-Concept or Clinical Tool?
Previous Article in Journal
Preparation of GST Inhibitor Nanoparticle Drug Delivery System and Its Reversal Effect on the Multidrug Resistance in Oral Carcinoma
Previous Article in Special Issue
Surface Wettability Modification of Cyclic Olefin Polymer by Direct Femtosecond Laser Irradiation
Article Menu

Export Article

Open AccessArticle
Nanomaterials 2015, 5(4), 1588-1609; doi:10.3390/nano5041588

Iron Oxide Nanoparticles Coated with a Phosphorothioate Oligonucleotide and a Cationic Peptide: Exploring Four Different Ways of Surface Functionalization

1
Université Paris 13, Sorbonne Paris Cité, UFR de Santé, Médecine et Biologie Humaine, F-93017 Bobigny, France
2
Université Paris Descartes, Laboratoire de Recherches Biochirurgicales, Hôpital Européen Georges Pompidou, F-75015 Paris, France
*
Author to whom correspondence should be addressed.
Academic Editor: Subramanian Tamil Selvan
Received: 18 August 2015 / Revised: 22 September 2015 / Accepted: 23 September 2015 / Published: 29 September 2015
(This article belongs to the Special Issue Nanoparticles in Theranostics)
View Full-Text   |   Download PDF [996 KB, uploaded 29 September 2015]   |  

Abstract

The superparamagnetic iron oxide nanoparticles (SPIONs) have great potential in therapeutic and diagnostic applications. Due to their superparamagnetic behavior, they are used clinically as a Magnetic Resonance Imaging (MRI) contrast agent. Iron oxide nanoparticles are also recognized todays as smart drug-delivery systems. However, to increase their specificity, it is essential to functionalize them with a molecule that effectively targets a specific area of the body. Among the molecules that can fulfill this role, peptides are excellent candidates. Oligonucleotides are recognized as potential drugs for various diseases but suffer from poor uptake and intracellular degradation. In this work, we explore four different strategies, based on the electrostatic interactions between the different partners, to functionalize the surface of SPIONs with a phosphorothioate oligonucleotide (ODN) and a cationic peptide labeled with a fluorophore. The internalization of the nanoparticles has been evaluated in vitro on RAW 264.7 cells. Among these strategies, the “«one-step assembly»”, i.e., the direct complexation of oligonucleotides and peptides on iron oxide nanoparticles, provides the best way of coating for the internalization of the nanocomplexes. View Full-Text
Keywords: iron oxide nanoparticles; peptide; oligonucleotide; surface functionalization iron oxide nanoparticles; peptide; oligonucleotide; surface functionalization
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Geinguenaud, F.; Banissi, C.; Carpentier, A.F.; Motte, L. Iron Oxide Nanoparticles Coated with a Phosphorothioate Oligonucleotide and a Cationic Peptide: Exploring Four Different Ways of Surface Functionalization. Nanomaterials 2015, 5, 1588-1609.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Nanomaterials EISSN 2079-4991 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top