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Nanomaterials 2015, 5(3), 1297-1316; doi:10.3390/nano5031297

Conjugation of Polymer-Coated Gold Nanoparticles with Antibodies—Synthesis and Characterization

1
Faculty of Science and Letters, Department of Biology, Aksaray University, Aksaray 68100,Turkey
2
Philipp University of Marburg, Marburg 35001, Germany
3
Centro de Investigación Cooperativa Biomagune, San Sebastián 20001, Spain
4
Faculty of Science, Department of Biology, Hacettepe University, Ankara 06800, Turkey
*
Authors to whom correspondence should be addressed.
Academic Editor: Subramanian Tamil Selvan
Received: 28 June 2015 / Revised: 27 July 2015 / Accepted: 30 July 2015 / Published: 7 August 2015
(This article belongs to the Special Issue Nanoparticles in Theranostics)
View Full-Text   |   Download PDF [3036 KB, uploaded 11 August 2015]   |  

Abstract

The synthesis of polymer-coated gold nanoparticles with high colloidal stability is described, together with appropriate characterization techniques concerning the colloidal properties of the nanoparticles. Antibodies against vascular endothelial growth factor (VEGF) are conjugated to the surface of the nanoparticles. Antibody attachment is probed by different techniques, giving a guideline about the characterization of such conjugates. The effect of the nanoparticles on human adenocarcinoma alveolar basal epithelial cells (A549) and human umbilical vein endothelial cells (HUVECs) is probed in terms of internalization and viability assays. View Full-Text
Keywords: gold nanoparticles; bioconjugation; nanoparticle characterization; toxicity; nanoparticle-cell interaction; cellular uptake; VEGF gold nanoparticles; bioconjugation; nanoparticle characterization; toxicity; nanoparticle-cell interaction; cellular uptake; VEGF
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Tan, G.; Kantner, K.; Zhang, Q.; Soliman, M.G.; del Pino, P.; Parak, W.J.; Onur, M.A.; Valdeperez, D.; Rejman, J.; Pelaz, B. Conjugation of Polymer-Coated Gold Nanoparticles with Antibodies—Synthesis and Characterization. Nanomaterials 2015, 5, 1297-1316.

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