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Nanomaterials 2015, 5(1), 284-297; doi:10.3390/nano5010284

DNA/Ag Nanoparticles as Antibacterial Agents against Gram-Negative Bacteria

Graduate School of Environmental Science, Hokkaido University, Hokkaido 060-0810, Japan
Nissei Bio Co. Ltd., Hokkaido 061-1374, Japan
Division of Materials Science and Engineering, Faculty of Engineering, Hokkaido University, Hokkaido 060-8628, Japan
Graduate School of Dental Medicine, Hokkaido University, Hokkaido 060-8586, Japan
Policy Alternative Research Institute, The University of Tokyo, Tokyo 113-0032, Japan
The present address of this author is: Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
Author to whom correspondence should be addressed.
Academic Editor: Thomas Nann
Received: 4 December 2014 / Revised: 20 February 2015 / Accepted: 25 February 2015 / Published: 3 March 2015
(This article belongs to the Special Issue Frontiers in Nucleic Acid Nanotechnology)
View Full-Text   |   Download PDF [1349 KB, uploaded 3 March 2015]   |  


Silver (Ag) nanoparticles were produced using DNA extracted from salmon milt as templates. Particles spherical in shape with an average diameter smaller than 10 nm were obtained. The nanoparticles consisted of Ag as the core with an outermost thin layer of DNA. The DNA/Ag hybrid nanoparticles were immobilized over the surface of cotton based fabrics and their antibacterial efficiency was evaluated using E. coli as the typical Gram-negative bacteria. The antibacterial experiments were performed according to the Antibacterial Standard of Japanese Association for the Functional Evaluation of Textiles. The fabrics modified with DNA/Ag nanoparticles showed a high enough inhibitory and killing efficiency against E. coli at a concentration of Ag ≥ 10 ppm. View Full-Text
Keywords: antibacterial; DNA/Ag nanoparticles; electrostatic interaction; Gram-negative bacteria; nanoparticle immobilization; salmon milt DNA antibacterial; DNA/Ag nanoparticles; electrostatic interaction; Gram-negative bacteria; nanoparticle immobilization; salmon milt DNA

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Takeshima, T.; Tada, Y.; Sakaguchi, N.; Watari, F.; Fugetsu, B. DNA/Ag Nanoparticles as Antibacterial Agents against Gram-Negative Bacteria. Nanomaterials 2015, 5, 284-297.

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