Next Article in Journal / Special Issue
Synthesis Characterization and Photocatalytic Studies of Cobalt Ferrite-Silica-Titania Nanocomposites
Previous Article in Journal
Reproductive Toxicity and Life History Study of Silver Nanoparticle Effect, Uptake and Transport in Arabidopsis thaliana
Previous Article in Special Issue
Evaluation of Superparamagnetic Silica Nanoparticles for Extraction of Triazines in Magnetic in-Tube Solid Phase Microextraction Coupled to Capillary Liquid Chromatography
Nanomaterials 2014, 4(2), 319-330; doi:10.3390/nano4020319

Hyperthermia Using Antibody-Conjugated Magnetic Nanoparticles and Its Enhanced Effect with Cryptotanshinone

1 Department of Electrical and Computer Engineering, Yokohama National University, Yokohama 240-8501, Japan 2 Department of Materials Science and Engineering, University of Washington, Seattle, WA 98195, USA
* Author to whom correspondence should be addressed.
Received: 17 February 2014 / Revised: 9 April 2014 / Accepted: 17 April 2014 / Published: 23 April 2014
(This article belongs to the Special Issue Magnetic Nanomaterials)
View Full-Text   |   Download PDF [1063 KB, uploaded 23 April 2014]   |   Browse Figures


Heat dissipation by magnetic nanoparticles (MNPs) under an alternating magnetic field can be used to selectively treat cancer tissues. Antibodies conjugated to MNPs can enhance the therapeutic effects of hyperthermia by altering antibody-antigen interactions. Fe3O4 nanoparticles (primary diameter, 20–30 nm) coated with polyethylenimine (PEI) were prepared and conjugated with CH11, an anti-Fas monoclonal antibody. HeLa cell growth was then evaluated as a function of antibody and MNP/antibody complex doses. HeLa cell growth decreased with increased doses of the antibody and complexes. However, MNPs alone did not affect cell growth; thus, only the antibody affected cell growth. In hyperthermia experiments conducted using an alternating magnetic field frequency of 210 kHz, cell viability varied with the intensity of the applied alternating magnetic field, because the temperature increase of the culture medium with added complexes was dependent on magnetic field intensity. The HeLa cell death rate with added complexes was significantly greater as compared with that with MNPs alone. Cryptotanshinone, an anti-apoptotic factor blocker, was also added to cell cultures, which provided an additional anti-cancer cell effect. Thus, an anti-cancer cell effect using a combination of magnetic hyperthermia, an anti-Fas antibody and cryptotanshinone was established.
Keywords: magnetic nanoparticles; hyperthermia; antibody; apoptosis; cryptotanshinone magnetic nanoparticles; hyperthermia; antibody; apoptosis; cryptotanshinone
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
MDPI and ACS Style

Ota, S.; Yamazaki, N.; Tomitaka, A.; Yamada, T.; Takemura, Y. Hyperthermia Using Antibody-Conjugated Magnetic Nanoparticles and Its Enhanced Effect with Cryptotanshinone. Nanomaterials 2014, 4, 319-330.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


Cited By

[Return to top]
Nanomaterials EISSN 2079-4991 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert