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J. Funct. Biomater. 2016, 7(2), 14; doi:10.3390/jfb7020014

Characterization of Tensile Mechanical Behavior of MSCs/PLCL Hybrid Layered Sheet

Interdisciplinary Graduate School of Engineering Sciences, Kyushu University, Fukuoka 816-8580, Japan
Fukuoka Dental Collage, Fukuoka 814-0193, Japan
Research Institute for Applied Mechanics, Kyushu University, Fukuoka 816-8580, Japan
Author to whom correspondence should be addressed.
Academic Editor: Daniel X. B. Chen
Received: 23 April 2016 / Revised: 19 May 2016 / Accepted: 27 May 2016 / Published: 3 June 2016
(This article belongs to the Special Issue Mechanical Properties of Tissue Engineering Scaffolds)
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A layered construct was developed by combining a porous polymer sheet and a cell sheet as a tissue engineered vascular patch. The primary objective of this study is to investigate the influence of mesenchymal stem cells (MSCs) sheet on the tensile mechanical properties of porous poly-(l-lactide-co-ε-caprolactone) (PLCL) sheet. The porous PLCL sheet was fabricated by the solid-liquid phase separation method and the following freeze-drying method. The MSCs sheet, prepared by the temperature-responsive dish, was then layered on the top of the PLCL sheet and cultured for 2 weeks. During the in vitro study, cellular properties such as cell infiltration, spreading and proliferation were evaluated. Tensile test of the layered construct was performed periodically to characterize the tensile mechanical behavior. The tensile properties were then correlated with the cellular properties to understand the effect of MSCs sheet on the variation of the mechanical behavior during the in vitro study. It was found that MSCs from the cell sheet were able to migrate into the PLCL sheet and actively proliferated into the porous structure then formed a new layer of MSCs on the opposite surface of the PLCL sheet. Mechanical evaluation revealed that the PLCL sheet with MSCs showed enhancement of tensile strength and strain energy density at the first week of culture which is characterized as the effect of MSCs proliferation and its infiltration into the porous structure of the PLCL sheet. New technique was presented to develop tissue engineered patch by combining MSCs sheet and porous PLCL sheet, and it is expected that the layered patch may prolong biomechanical stability when implanted in vivo. View Full-Text
Keywords: cell sheet technology; scaffold tissue engineering; PLCL sheet; MSCs sheet; tensile mechanical properties; tissue engineered patch cell sheet technology; scaffold tissue engineering; PLCL sheet; MSCs sheet; tensile mechanical properties; tissue engineered patch

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Pangesty, A.I.; Arahira, T.; Todo, M. Characterization of Tensile Mechanical Behavior of MSCs/PLCL Hybrid Layered Sheet. J. Funct. Biomater. 2016, 7, 14.

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J. Funct. Biomater. EISSN 2079-4983 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
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